CaltechAUTHORS
  A Caltech Library Service

A New Chamber for Studying the Behavior of Drosophila

Simon, Jasper C. and Dickinson, Michael H. (2010) A New Chamber for Studying the Behavior of Drosophila. PLoS ONE, 5 (1). e8793. ISSN 1932-6203. PMCID PMC2811731. https://resolver.caltech.edu/CaltechAUTHORS:20100225-094228460

[img]
Preview
PDF - Published Version
Creative Commons Attribution.

2492Kb
[img]
Preview
Image (TIFF) (Figure S1) - Supplemental Material
Creative Commons Attribution.

591Kb
[img]
Preview
Image (TIFF) (Figure S2) - Supplemental Material
Creative Commons Attribution.

3468Kb
[img]
Preview
Image (TIFF) (Figure S3) - Supplemental Material
Creative Commons Attribution.

5Mb
[img] Video (AVI) (Movie S1) - Supplemental Material
Creative Commons Attribution.

22Mb
[img] Video (AVI) (Movie S2) - Supplemental Material
Creative Commons Attribution.

2131Kb
[img] Video (AVI) (Movie S3) - Supplemental Material
Creative Commons Attribution.

2633Kb
[img] Video (AVI) (Movie S4) - Supplemental Material
Creative Commons Attribution.

2533Kb
[img] Video (AVI) (Movie S5) - Supplemental Material
Creative Commons Attribution.

3190Kb
[img] Video (AVI) (Movie S6) - Supplemental Material
Creative Commons Attribution.

3409Kb
[img] Video (AVI) (Movie S7) - Supplemental Material
Creative Commons Attribution.

2136Kb
[img] Video (AVI) (Movie S8) - Supplemental Material
Creative Commons Attribution.

779Kb
[img] Video (AVI) (Movie S9) - Supplemental Material
Creative Commons Attribution.

776Kb
[img] Video (AVI) (Movie S10) - Supplemental Material
Creative Commons Attribution.

668Kb
[img] Video (AVI) (Movie S11) - Supplemental Material
Creative Commons Attribution.

659Kb
[img] Video (AVI) (Movie S12) - Supplemental Material
Creative Commons Attribution.

362Kb
[img] Video (AVI) (Movie S13) - Supplemental Material
Creative Commons Attribution.

1952Kb
[img] Video (AVI) (Movie S14) - Supplemental Material
Creative Commons Attribution.

608Kb
[img] Video (AVI) (Movie S15) - Supplemental Material
Creative Commons Attribution.

378Kb
[img] Video (AVI) (Movie S16) - Supplemental Material
Creative Commons Attribution.

1107Kb
[img] Video (AVI) (Movie S17) - Supplemental Material
Creative Commons Attribution.

1471Kb
[img] Video (AVI) (Movie S18) - Supplemental Material
Creative Commons Attribution.

1271Kb
[img] Video (AVI) (Movie S19) - Supplemental Material
Creative Commons Attribution.

579Kb
[img] Video (AVI) (Movie S20) - Supplemental Material
Creative Commons Attribution.

1108Kb
[img] Video (AVI) (Movie S21) - Supplemental Material
Creative Commons Attribution.

1123Kb
[img] Video (AVI) (Movie S22) - Supplemental Material
Creative Commons Attribution.

634Kb
[img] Video (AVI) (Movie S23) - Supplemental Material
Creative Commons Attribution.

360Kb
[img] Video (AVI) (Movie S24) - Supplemental Material
Creative Commons Attribution.

471Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20100225-094228460

Abstract

Methods available for quickly and objectively quantifying the behavioral phenotypes of the fruit fly, Drosophila melanogaster, lag behind in sophistication the tools developed for manipulating their genotypes. We have developed a simple, easy-to-replicate, general-purpose experimental chamber for studying the ground-based behaviors of fruit flies. The major innovative feature of our design is that it restricts flies to a shallow volume of space, forcing all behavioral interactions to take place within a monolayer of individuals. The design lessens the frequency that flies occlude or obscure each other, limits the variability in their appearance, and promotes a greater number of flies to move throughout the center of the chamber, thereby increasing the frequency of their interactions. The new chamber design improves the quality of data collected by digital video and was conceived and designed to complement automated machine vision methodologies for studying behavior. Novel and improved methodologies for better quantifying the complex behavioral phenotypes of Drosophila will facilitate studies related to human disease and fundamental questions of behavioral neuroscience.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0008793DOIArticle
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0008793PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811731/PubMed CentralArticle
ORCID:
AuthorORCID
Dickinson, Michael H.0000-0002-8587-9936
Additional Information:© 2010 Simon, Dickinson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: November 6, 2009; accepted: December 2, 2009; published: January 27, 2010. Editor: Kenji Hashimoto, Chiba University Center for Forensic Mental Health, Japan. Funding for this research was provided by United States National Institutes of Health grant 320 R01 DA022777 (to MHD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Kristin Branson, Andrew Straw, and Heiko Dankert for council regarding the use of their software. Pietro Perona and Peter Polidoro contributed to the design of early chambers. We thank Mike Vondrus and Peter Polidoro for help machining early prototypes of the new chamber. David Anderson and members of his laboratory, Liming Wang and Kiichi Watanabe, provided space, advice, and the CS strain for the courtship and aggression trials. We thank Kristin Branson for help producing the supplementary movies. Author contributions: Conceived and designed the experiments: JCS. Performed the experiments: JCS. Analyzed the data: JCS. Wrote the paper: JCS MD. Conceived and developed the technology: JCS.
Funders:
Funding AgencyGrant Number
NIH320 R01 DA022777
Issue or Number:1
PubMed Central ID:PMC2811731
Record Number:CaltechAUTHORS:20100225-094228460
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100225-094228460
Official Citation: Simon JC, Dickinson MH (2010) A New Chamber for Studying the Behavior of Drosophila. PLoS ONE 5(1): e8793. doi:10.1371/journal.pone.0008793
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17591
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:25 Feb 2010 20:42
Last Modified:03 Oct 2019 01:30

Repository Staff Only: item control page