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Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication

Kumagai, Akiko and Shevchenko, Anna and Shevchenko, Andrej and Dunphy, William G. (2010) Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication. Cell, 140 (3). pp. 349-359. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20100303-105920203

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Abstract

TopBP1 has important roles in both DNA replication and checkpoint regulation in vertebrates. We have identified a protein called Treslin that associates with TopBP1 in Xenopus egg extracts. Depletion of Treslin from egg extracts strongly inhibits chromosomal DNA replication. Binding of Treslin to chromatin in egg extracts occurs independently of TopBP1. However, loading of the initiator protein Cdc45 onto chromatin cannot take place in the absence of Treslin. Prior to the initiation of DNA replication, Treslin associates with TopBP1 in a Cdk2-dependent manner. Ablation of Treslin from human cells also strongly inhibits DNA replication. Taken together, these results indicate that Treslin and TopBP1 collaborate in the Cdk2-mediated loading of Cdc45 onto replication origins. Thus, Treslin regulates a pivotal step in the initiation of DNA replication in vertebrates.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cell.2009.12.049DOIUNSPECIFIED
http://linkinghub.elsevier.com/retrieve/pii/S0092867409016353PublisherUNSPECIFIED
http://www.cell.com/retrieve/pii/S0092867409016353PublisherUNSPECIFIED
ORCID:
AuthorORCID
Kumagai, Akiko0000-0003-2422-8053
Dunphy, William G.0000-0001-7598-8939
Additional Information:© 2010 Elsevier Inc. Received 28 May 2009; revised 14 October 2009; accepted 22 December 2009. Published online: January 28, 2010. Available online 28 January 2010. We are grateful to laboratory members for comments on the manuscript. We would also like to thank Rochelle Diamond for assistance with the FACS analyses and T.C. Spelsberg (Mayo Clinic) for the U2OS T-REx cells. This work was supported by National Institutes of Health grants GM043974 and GM070891 to W.G.D.
Funders:
Funding AgencyGrant Number
NIHGM043974
NIHGM070891
Subject Keywords:CELLCYCLE; DNA
Issue or Number:3
Record Number:CaltechAUTHORS:20100303-105920203
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100303-105920203
Official Citation:Akiko Kumagai, Anna Shevchenko, Andrej Shevchenko, William G. Dunphy, Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication, Cell, Volume 140, Issue 3, 5 February 2010, Pages 349-359, ISSN 0092-8674, DOI: 10.1016/j.cell.2009.12.049. (http://www.sciencedirect.com/science/article/B6WSN-4Y89YXH-1/2/38506481d6f05d0b539f19a43896c4e5)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17647
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Mar 2010 18:49
Last Modified:09 Mar 2020 13:18

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