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Thermal Monitoring: Raman Spectrometer System for Remote Measurement of Cellular Temperature on a Microscopic Scale

Pikov, Victor and Siegel, Peter H. (2010) Thermal Monitoring: Raman Spectrometer System for Remote Measurement of Cellular Temperature on a Microscopic Scale. IEEE Engineering in Medicine and Biology Magazine, 29 (1). pp. 63-71. ISSN 0739-5175.

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A simple setup was demonstrated for remote temperature monitoring of water, water-based media, and cells on a microscopic scale. The technique relies on recording changes in the shape of a stretching band of the hydroxyl group in liquid water at 3,100-3,700 cm^(-1). Rather than direct measurements in the near-infrared (IR), a simple Raman spectrometer setup was realized. The measured Raman shifts were observed at near optical wavelengths using an inverted microscope with standard objectives in contrast to costly near-IR elements. This allowed for simultaneous visible inspection through the same optical path. An inexpensive 671-nm diode pump laser (<100 mW), standard dichroic and lowpass filters, and a commercial 600-1,000 nm spectrometer complete the instrument.

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Siegel, Peter H.0000-0002-2539-4646
Additional Information:© 2010 IEEE. The authors gratefully acknowledge the contributions of Ms. Kathleen Teves, a summer undergraduate research assistant from the University of California (UC) Irvine, for collecting the measurements data and the continuing support of Caltech Profs. David B. Rutledge and Scott E. Fraser. They also thank Dr. Alex Sigel for the H1299 cell line, and they especially thank Mr. Jay Moskovic for the loan of the Andor spectrometer. This work was carried out under grants from the National Institutes of Health.
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Record Number:CaltechAUTHORS:20100316-091641985
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17746
Deposited By: Tony Diaz
Deposited On:22 Mar 2010 03:52
Last Modified:09 Mar 2020 13:19

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