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Multiplexed DNA-Modified Electrodes

Slinker, Jason D. and Muren, Natalie B. and Gorodetsky, Alon A. and Barton, Jacqueline K. (2010) Multiplexed DNA-Modified Electrodes. Journal of the American Chemical Society, 132 (8). pp. 2769-2774. ISSN 0002-7863. PMCID PMC3334307. doi:10.1021/ja909915m. https://resolver.caltech.edu/CaltechAUTHORS:20100324-111156290

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Abstract

We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets. Four DNA sequences were simultaneously distinguished on a single DME chip with 4-fold redundancy, including one incorporating a single base mismatch. These chips also enabled investigation of the sequence-specific activity of the restriction enzyme Alu1. DME chips supported dense DNA monolayer formation with high reproducibility, as confirmed by statistical comparison to commercially available rod electrodes. The working electrode areas on the chips were reduced to 10 μm in diameter, revealing microelectrode behavior that is beneficial for high sensitivity and rapid kinetic analysis. These results illustrate how DME chips facilitate sensitive and selective detection of DNA and DNA-binding protein targets in a robust and internally standardized multiplexed format.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja909915mDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja909915mPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334307/PubMed CentralArticle
ORCID:
AuthorORCID
Gorodetsky, Alon A.0000-0002-3811-552X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2010 American Chemical Society. Received November 23, 2009. Publication Date (Web): February 4, 2010. This research was supported by the NIH (GM61077). J.D.S. also thanks the National Institute of Biomedical Imaging and Bioengineering for a postdoctoral fellowship. The authors thank J. Genereux, M. Buzzeo, and D. Ceres for fruitful discussions, J. DeFranco for assistance with photolithography. and S. Olson and M. Roy for construction of the test mount and clamp assembly.
Funders:
Funding AgencyGrant Number
NIHGM61077
National Institute of Biomedical Imaging and BioengineeringUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:8
PubMed Central ID:PMC3334307
DOI:10.1021/ja909915m
Record Number:CaltechAUTHORS:20100324-111156290
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100324-111156290
Official Citation:Multiplexed DNA-Modified Electrodes Jason D. Slinker, Natalie B. Muren, Alon A. Gorodetsky, Jacqueline K. Barton Journal of the American Chemical Society 2010 132 (8), 2769-2774
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17788
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:29 Mar 2010 22:34
Last Modified:08 Nov 2021 23:38

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