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Interfacial Reactions of Ozone with Surfactant Protein B in a Model Lung Surfactant System

Kim, Hugh I. and Kim, Hyungjun and Shin, Young Shik and Beegle, Luther W. and Jang, Seung Soon and Neidholdt, Evan L. and Goddard, William A., III and Heath, James R. and Kanik, Isik and Beauchamp, J. L. (2010) Interfacial Reactions of Ozone with Surfactant Protein B in a Model Lung Surfactant System. Journal of the American Chemical Society, 132 (7). pp. 2254-2263. ISSN 0002-7863. PMCID PMC2830728. http://resolver.caltech.edu/CaltechAUTHORS:20100407-112655335

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Abstract

Oxidative stresses from irritants such as hydrogen peroxide and ozone (O_3) can cause dysfunction of the pulmonary surfactant (PS) layer in the human lung, resulting in chronic diseases of the respiratory tract. For identification of structural changes of pulmonary surfactant protein B (SP-B) due to the heterogeneous reaction with O_3, field-induced droplet ionization (FIDI) mass spectrometry has been utilized. FIDI is a soft ionization method in which ions are extracted from the surface of microliter-volume droplets. We report structurally specific oxidative changes of SP-B_(1−25) (a shortened version of human SP-B) at the air−liquid interface. We also present studies of the interfacial oxidation of SP-B_(1−25) in a nonionizable 1-palmitoyl-2-oleoyl-sn-glycerol (POG) surfactant layer as a model PS system, where competitive oxidation of the two components is observed. Our results indicate that the heterogeneous reaction of SP-B_(1−25) at the interface is quite different from that in the solution phase. In comparison with the nearly complete homogeneous oxidation of SP-B_(1−25), only a subset of the amino acids known to react with ozone are oxidized by direct ozonolysis in the hydrophobic interfacial environment, both with and without the lipid surfactant layer. Combining these experimental observations with the results of molecular dynamics simulations provides an improved understanding of the interfacial structure and chemistry of a model lung surfactant system subjected to oxidative stress.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja908477w DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja908477wPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830728/PubMed CentralArticle
ORCID:
AuthorORCID
Goddard, William A., III0000-0003-0097-5716
Heath, James R.0000-0001-5356-4385
Beauchamp, J. L.0000-0001-8839-4822
Additional Information:© 2010 American Chemical Society. Received October 5, 2009. Publication Date (Web): February 2, 2010. The research described in this paper was carried out at the Beckman Institute and the Noyes Laboratory of Chemical Physics at the California Institute of Technology, the Computational NanoBio Technology Laboratory at Georgia Institute of Technology, and the Jet Propulsion Laboratory under a contract with the National Aeronautics and Space Administration and funded through the Director’s Research and Development Fund. We gratefully acknowledge financial support provided by National Science Foundation (NSF) under Grant CHE-0416381 (J.L.B., PI) and the Beckman Institute Mass Spectrometry Resource Center. Y.S.S. and J.R.H. acknowledge the support of the National Cancer Institute under Grant 5U54 CA119347 (J.R.H., PI).
Funders:
Funding AgencyGrant Number
NSFCHE-0416381
NIH5U54 CA119347
PubMed Central ID:PMC2830728
Record Number:CaltechAUTHORS:20100407-112655335
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100407-112655335
Official Citation:Interfacial Reactions of Ozone with Surfactant Protein B in a Model Lung Surfactant System Hugh I. Kim, Hyungjun Kim, Young Shik Shin, Luther W. Beegle, Seung Soon Jang, Evan L. Neidholdt, William A. Goddard, James R. Heath, Isik Kanik, J. L. Beauchamp Journal of the American Chemical Society 2010 132 (7), 2254-2263
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17886
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Apr 2010 18:32
Last Modified:01 Sep 2017 20:33

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