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Arabidopsis Regeneration from Multiple Tissues Occurs via a Root Development Pathway

Sugimoto, Kaoru and Jiao, Yuling and Meyerowitz, Elliot M. (2010) Arabidopsis Regeneration from Multiple Tissues Occurs via a Root Development Pathway. Developmental Cell, 18 (3). pp. 463-471. ISSN 1534-5807. http://resolver.caltech.edu/CaltechAUTHORS:20100412-083057524

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Abstract

Unlike most animal cells, plant cells can easily regenerate new tissues from a wide variety of organs when properly cultured. The common elements that provide varied plant cells with their remarkable regeneration ability are still largely unknown. Here we describe the initial process of Arabidopsis in vitro regeneration, where a pluripotent cell mass termed callus is induced. We demonstrate that callus resembles the tip of a root meristem, even if it is derived from aerial organs such as petals, which clearly shows that callus formation is not a simple reprogramming process backward to an undifferentiated state as widely believed. Furthermore, callus formation in roots, cotyledons, and petals is blocked in mutant plants incapable of lateral root initiation. It thus appears that the ectopic activation of a lateral root development program is a common mechanism in callus formation from multiple organs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.devcel.2010.02.004 DOIArticle
http://www.sciencedirect.com/science/article/pii/S1534580710000924PublisherArticle
ORCID:
AuthorORCID
Jiao, Yuling0000-0002-1189-1676
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2010 Elsevier Inc. Received 21 September 2009; revised 16 December 2009; accepted 3 February 2010. Published: March 15, 2010. Available online 15 March 2010. We thank B. Scheres for the pWOX5::GFPER seeds, J. Schiefelbein for the pGL2::GFPER seeds, P.N. Benfey for the pSCR::GFPER and pSHR::SHRGFP seeds, and J.L. Celenza, Jr. for the alf4-1 seeds. We thank S. Gordon for the help and valuable comments in setting up this project, V. Rao for advice on microarray analysis, A. Garda for technical assistance and advice, and J. Harrison, C. Peterson, and members of Meyerowitz lab for critical reading and comments on the manuscript. We are grateful to A. Roeder for her continuous support and all her suggestions on this project. This research was supported by a Gosney Postdoctoral Fellowship and grant IOS-0846192 from the National Science Foundation.
Funders:
Funding AgencyGrant Number
Caltech Gosney FellowshipUNSPECIFIED
NSFIOS-0846192
Subject Keywords:DEVBIO; SIGNALING; STEMCELL
Record Number:CaltechAUTHORS:20100412-083057524
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100412-083057524
Official Citation:Kaoru Sugimoto, Yuling Jiao, Elliot M. Meyerowitz, Arabidopsis Regeneration from Multiple Tissues Occurs via a Root Development Pathway, Developmental Cell, Volume 18, Issue 3, 16 March 2010, Pages 463-471, ISSN 1534-5807, http://dx.doi.org/10.1016/j.devcel.2010.02.004. (http://www.sciencedirect.com/science/article/pii/S1534580710000924)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17924
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2010 16:27
Last Modified:10 Dec 2016 22:16

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