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Orchestration of Floral Initiation by APETALA1

Kaufmann, Kerstin and Wellmer, Frank and Muiño, Jose M. and Ferrier, Thilia and Wuest, Samuel E. and Kumar, Vijaya and Serrano-Mislata, Antonio and Madueño, Francisco and Krajewski, Pawel and Meyerowitz, Elliot M. and Angenent, Gerco C. and Riechmann, José Luis (2010) Orchestration of Floral Initiation by APETALA1. Science, 328 (5974 ). pp. 85-89. ISSN 0036-8075. doi:10.1126/science.1185244. https://resolver.caltech.edu/CaltechAUTHORS:20100412-093513319

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Abstract

The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using a combination of gene expression profiling and genome-wide binding studies. Many of its targets encode transcriptional regulators, including known floral repressors. The latter genes are down-regulated by AP1, suggesting that it initiates floral development by abrogating the inhibitory effects of these genes. Although AP1 acts predominantly as a transcriptional repressor during the earliest stages of flower development, at more advanced stages it also activates regulatory genes required for floral organ formation, indicating a dynamic mode of action. Our results further imply that AP1 orchestrates floral initiation by integrating growth, patterning, and hormonal pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1185244 DOIArticle
http://www.sciencemag.org/cgi/content/abstract/328/5974/85PublisherArticle
http://www.sciencemag.org/cgi/content/full/328/5974/85/DC1PublisherSupporting Material
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2010 American Association for the Advancement of Science. Received 26 November 2009; accepted 4 March 2010. We are grateful to T. Mastro for help with microarray hybridizations, S. Kushnir for generating AP1 antibodies, Y. Hanzawa and D. Bradley for providing transferred DNA (T-DNA) insertion lines, and E. Graciet for help with AP1-GR detection in nuclear extracts. This work was supported by grants from Science Foundation Ireland (06/IN.1/B851 to F.W.), European Union (EU)–Marie Curie program (Transistor-MRTNCT-2004–512285 to G.C.A. and IRG-224864 to J.L.R.), NSF (2010-0520193 to J.L.R. and E.M.M.), Spanish Ministerio de Ciencia e Innovación (BFU2008-04251 to J.L.R. and BIO2006-10994 and BIO2009-10876 to F.M.), and by the Millard and Muriel Jacobs Laboratory at Caltech. G.C.A. was also supported by the Netherlands Proteomics Centre and CBSG, which are part of the Netherlands Genomic Initiative. K.K. and J.M.M. were supported by fellowships from the EU–Marie Curie program; J.M.M. was also supported by CBSG and a Horizon grant (#93519020). T.F. was supported by a fellowship from CRAG. Microarray data have been deposited with the National Center for Biotechnology Information Gene Expression Omnibus under accession numbers GSE20184 and GSE20138 and the sequencing data under accession number GSE20176.
Funders:
Funding AgencyGrant Number
Science Foundation, Ireland06/IN.1/B851
European Union (EU)Transistor-MRTNCT-2004-512285
European Union (EU)IRG-224864
NSF2010-0520193
Ministerio de Ciencia e Innovacion (MCINN)BFU2008-04251
Ministerio de Ciencia e Innovacion (MCINN)BIO2006-10994
Ministerio de Ciencia e Innovacion (MCINN)BIO2009-10876
Caltech Millard and Muriel Jacobs Genetics and Genomics LaboratoryUNSPECIFIED
Netherlands Proteomics CentreUNSPECIFIED
CBSGUNSPECIFIED
Horizon93519020
CRAGUNSPECIFIED
Issue or Number:5974
DOI:10.1126/science.1185244
Record Number:CaltechAUTHORS:20100412-093513319
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100412-093513319
Official Citation:Orchestration of Floral Initiation by APETALA1 BY KERSTIN KAUFMANN, FRANK WELLMER, JOSE M. MUIÑO, THILIA FERRIER, SAMUEL E. WUEST, VIJAYA KUMAR, ANTONIO SERRANO-MISLATA, FRANCISCO MADUEÑO, PAWEL KRAJEWSKI, ELLIOT M. MEYEROWITZ, GERCO C. ANGENENT, JOSÉ LUIS RIECHMANN Science 02 Apr 2010: Vol. 328, Issue 5974, pp. 85-89 DOI: 10.1126/science.1185244
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17931
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2010 19:31
Last Modified:08 Nov 2021 23:40

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