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Fluorescent labeling, sensing, and differentiation of leukocytes from undiluted whole blood samples

Zheng, Siyang and Lin, Jeffrey Chun-Hui and Kasdan, Harvey L. and Tai, Yu-Chong (2008) Fluorescent labeling, sensing, and differentiation of leukocytes from undiluted whole blood samples. Sensors and Actuators B, 132 (2). pp. 558-567. ISSN 0925-4005. doi:10.1016/j.snb.2007.11.031.

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In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices.A challenging issue in leukocyte sensing from blood samples is the required high-dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye acridine orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525 nm is used for leukocyte count and red fluorescent signal centered at 650 nm is used for leukocyte differentiation. Throughput of 1000 leukocytes per second was achieved.

Item Type:Article
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Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2007 Elsevier B.V. Available online 22 November 2007. This work is supported by the National Space Biomedical Research Institute through NASA NCC 9-58. The authors would like to thank other members of the Caltech Micromachining Laboratory for their valuable assistance.
Funding AgencyGrant Number
Subject Keywords:Leukocyte differential; Fluorescent sensing; Acridine orange
Issue or Number:2
Record Number:CaltechAUTHORS:20100511-132714107
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18241
Deposited By: Ruth Sustaita
Deposited On:11 May 2010 21:01
Last Modified:08 Nov 2021 23:42

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