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A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Liu, Zhenfeng and Walton, Troy A. and Rees, Douglas C. (2010) A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators. Protein Science, 19 (4). pp. 808-814. ISSN 0961-8368. PMCID PMC2867020. http://resolver.caltech.edu/CaltechAUTHORS:20100514-150930270

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Abstract

Several archaeal mechanosensitive (MS) channels have been reported, including one from Thermoplasma volcanium designated MscTV. Here, we report the crystal structure of MscTV at 1.6-Å resolution. Unexpectedly, MscTV was found to be a water-soluble protein exhibiting a winged helix-turn-helix (wHTH) motif, which is the signature of the MarR (multiple antibiotic resistance regulator) family of transcriptional regulators. A cell-based osmotic downshock functional assay demonstrated that MscTV was unable to protect a knockout strain of Escherichia coli from hypoosmotic shock, further indicating that it does not function as a MS channel. We propose this protein be renamed MLPTv for MarR-like protein from T. volcanium.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/pro.360 DOIArticle
http://www3.interscience.wiley.com/journal/123283443/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2867020/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0002-1076-1503
Additional Information:© 2010 The Protein Society. Received: 27 December 2009; Revised: 23 January 2010; Accepted: 25 January 2010. The authors thank A. Shih for cloning work, Y. Poon and J. Lai for protocols of osmotic downshock assay and lysogenizing MJF465 cells with kDE3, Y. Liu and M. Shahgholi for the mass spectrometry analysis, B. Martinac, H. Pinkett, and C. Gandhi for discussions, P. Blount for the MJF465 cells, and the staff at SSRL for technical support during data collection. The authors acknowledge the Gordon and Betty Moore Foundation for support of the Molecular Observatory at Caltech. Operations at SSRL are supported by the U.S. Department of Energy and NIH. D. C. R. is an Investigator in the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIHGM084211
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Subject Keywords:mechanosensitive channel; multiple antibiotic resistance regulator; transcriptional regulator; winged helix-turn-helix motif
PubMed Central ID:PMC2867020
Record Number:CaltechAUTHORS:20100514-150930270
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100514-150930270
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18314
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Jun 2010 18:56
Last Modified:13 Mar 2015 19:40

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