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Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method

Zhang, Xiaowei and Bearer, Elaine L. and Perles-Barbacaru, Adriana T. and Jacobs, Russell E. (2010) Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method. Magnetic Resonance in Medicine, 63 (5). pp. 1391-1397. ISSN 1084-7529. PMCID PMC3387550. https://resolver.caltech.edu/CaltechAUTHORS:20100521-152642118

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Abstract

Golgi impregnation is unique in its ability to display the dendritic trees and axons of large numbers of individual neurons by histology. Here we apply magnetic resonance microscopy to visualize the neuroanatomy of animal models by combining histologic fixation chemistry with paramagnetic contrast agents. Although there is some differential uptake of the standard small-molecular-weight contrast agents by different tissue types, detailed discrimination of tissue architecture in MR images does not approach that of standard histology. Our modified Golgi impregnation method significantly increases anatomic detail in magnetic resonance microscopy images. Fixed mouse brains were treated with a solution containing a paramagnetic contrast agent (gadoteridol) and potassium dichromate. Results demonstrate a specific contrast enhancement likely due to diamagnetic hexavalent chromium undergoing tissue specific reduction to paramagnetic trivalent chromium. This new method dramatically improves neuroanatomical contrast compared to conventional fixation, displaying detail approximating that of histologic specimens at low (4×) magnification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/mrm.22322 DOIArticle
http://www3.interscience.wiley.com/journal/123375987/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387550/PubMed CentralArticle
ORCID:
AuthorORCID
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 2010 Wiley. Received: 31 March 2009; Revised: 10 November 2009; Accepted: 13 November 2009. We are grateful for the technical assistance of Paulette Ferland, Bryan Kinney, and Neuroscience Associates for help with the histologic preparations. This project was funded in part by the Beckman Institute (R.E.J.), NIH NIGMS GM47368, NINDS NS046810 (E.L.B.), NIDA R01DA18184, and NCRR U24 RR021760 Mouse BIRN (R.E.J.).
Funders:
Funding AgencyGrant Number
Caltech Beckman InstituteUNSPECIFIED
NIHGM47368
National Institute of Neurological Disorders and Stroke (NINDS)NS046810
National Institute on Drug AbuseR01DA18184
National Center for Research Resources (NCRR)U24 RR021760
Subject Keywords:Contrast agent; Golgi stain; neuroanatomy; mouse; microMRI; histology
Issue or Number:5
PubMed Central ID:PMC3387550
Record Number:CaltechAUTHORS:20100521-152642118
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100521-152642118
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18402
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:24 May 2010 21:24
Last Modified:03 Oct 2019 01:42

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