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Molecular robots guided by prescriptive landscapes

Lund, Kyle and Manzo, Anthony J. and Dabby, Nadine and Michelotti, Nicole and Johnson-Buck, Alexander and Nangreave, Jeanette and Taylor, Steven and Pei, Renjun and Stojanovic, Milan N. and Walter, Nils G. and Winfree, Erik and Yan, Hao (2010) Molecular robots guided by prescriptive landscapes. Nature, 465 (7295). pp. 206-210. ISSN 0028-0836. doi:10.1038/nature09012.

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Traditional robots rely for their function on computing, to store internal representations of their goals and environment and to coordinate sensing and any actuation of components required in response. Moving robotics to the single-molecule level is possible in principle, but requires facing the limited ability of individual molecules to store complex information and programs. One strategy to overcome this problem is to use systems that can obtain complex behaviour from the interaction of simple robots with their environment. A first step in this direction was the development of DNA walkers, which have developed from being non-autonomous, to being capable of directed but brief motion on one-dimensional tracks. Here we demonstrate that previously developed random walkers—so-called molecular spiders that comprise a streptavidin molecule as an inert ‘body’ and three deoxyribozymes as catalytic ‘legs’—show elementary robotic behaviour when interacting with a precisely defined environment. Single-molecule microscopy observations confirm that such walkers achieve directional movement by sensing and modifying tracks of substrate molecules laid out on a two-dimensional DNA origami landscape. When using appropriately designed DNA origami, the molecular spiders autonomously carry out sequences of actions such as ‘start’, ‘follow’, ‘turn’ and ‘stop’. We anticipate that this strategy will result in more complex robotic behaviour at the molecular level if additional control mechanisms are incorporated. One example might be interactions between multiple molecular robots leading to collective behaviour; another might be the ability to read and transform secondary cues on the DNA origami landscape as a means of implementing Turing-universal algorithmic behaviour.

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Winfree, Erik0000-0002-5899-7523
Additional Information:© 2010 Macmillan Publishers Limited. Received 31 March 2009. Accepted 11 March 2010. This research was supported by the US National Science Foundation (NSF) EMT and CBC grants (all authors); fellowships and grants from the Kinship Foundation (Searle), the Leukemia & Lymphoma Society, the Juvenile Diabetes Research Foundation and NSF ITR (M.N.S.); awards from the US Army Research Office, the NSF, the US Office of Naval Research, the US National Institutes of Health, the US Department of Energy and a Sloan Research Fellowship (H.Y.); an NSF Graduate Fellowship (N.D.); and Molecular Biophysics and Microfluidics in Biomedical Sciences Training Fellowships from the NIH (A.J.-B. and N.M., respectively). M.N.S. is grateful to T. E. Mitchell and M. Olah for inspiration, discussions and help. Author Contributions: AFM experiments were performed by K.L. (majority), J.N. and N.D.; analysis was performed by N.D., K.L., J.N. and S.T. and supervised by E.W. and H.Y. Fluorescence microscopy and particle tracking analysis were performed by A.J.M., N.M. and A.J.-B, supervised by N.G.W. Spiders were synthesized and purified, and their integrity was confirmed and monitored, by S.T. Surface plasmon resonance experiments were performed by R.P. Research coordination was by M.N.S. and materials transfer coordination was by S.T., J.N. and K.L. Experimental design and manuscript preparation received input from all authors.
Funding AgencyGrant Number
Kinship Foundation (Searle)UNSPECIFIED
Leukemia & Lymphoma SocietyUNSPECIFIED
Juvenile Diabetes Research FoundationUNSPECIFIED
Army Research Office (ARO)UNSPECIFIED
Office of Naval Research (ONR)UNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:biochemistry
Issue or Number:7295
Record Number:CaltechAUTHORS:20100602-145349454
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18532
Deposited By: Tony Diaz
Deposited On:18 Jun 2010 17:48
Last Modified:08 Nov 2021 23:44

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