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Cardiopulmonary response to inhalation of biogenic secondary organic aerosol

McDonald, Jacob D. and Doyle-Eisele, Melanie and Campen, Matthew J. and Seagrave, JeanClare and Holmes, Tom and Lund, Amie and Surratt, Jason D. and Seinfeld, John H. and Rohr, Annette C. and Knipping, Eladio M. (2010) Cardiopulmonary response to inhalation of biogenic secondary organic aerosol. Inhalation Toxicology, 22 (3). pp. 253-265. ISSN 0895-8378.

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An irradiation chamber designed for reproducible generation of inhalation test atmospheres of secondary organic aerosol (SOA) was used to evaluate cardiopulmonary responses in rodents exposed to SOA derived from the oxidation of α-pinene. SOA atmospheres were produced with 10:1 ratios of α-pinene:nitrogen oxides (NO_x) and 10:1:1 ratios of α-pinene:nitrogen oxides:sulfur dioxide (SO_2). SOA atmospheres were produced to yield 200 μg m^(−3) of particulate matter (PM). Exposures were conducted downstream of honeycomb denuders employed to remove the gas-phase precursors and reaction products. Nose-only exposures were conducted with both rats (pulmonary effects) and mice (pulmonary and cardiovascular effects). Composition of the atmospheres was optimized to ensure that the SOA generated resembled SOA observed in previous irradiation studies, and contained specific SOA compounds of interest (e.g., organosulfates) identified in ambient air. Pulmonary and cardiovascular toxicity were measured in two different rodent species. In situ chemiluminescence and thiobarbituric acid– reactive substances (TBARS) were used to evaluate oxidative reactions in the F344 rats. ApoE^(−/−) mice were exposed for 7 days and measurements of TBARS and gene expression of heme oxygenase-1 (HO-1), endothelin-1 (ET-1), matrix metalloproteinase-9 (MMP-9) were made in aorta. Pulmonary inflammatory responses in both species were measured by bronchoalveolar lavage fluid (BALF) cell counts. No pulmonary inflammation was observed in either species. A mild response was observed in mouse aorta for the upregulation of HO-1 and MMP-9, but was not seen for ET-1. Overall, α-pinene–derived SOA, including SOA that included organosulfate compounds, revealed limited biological response after short-term inhalation exposures.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle
Surratt, Jason D.0000-0002-6833-1450
Seinfeld, John H.0000-0003-1344-4068
Additional Information:© 2010 Informa UK Ltd. Received 28 April 2009; revised 23 June 2009; accepted 26 June 2009. This research was sponsored by the Electric Power Research Institute under Contract EP-P20421/C9942/061961.
Funding AgencyGrant Number
Electric Power Research Institute (EPRI)EP-P20421/C9942/061961
Subject Keywords:secondary organic aerosol, air pollution, vascular toxicity
Issue or Number:3
Record Number:CaltechAUTHORS:20100607-085410213
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18577
Deposited By: Tony Diaz
Deposited On:23 Jun 2010 18:01
Last Modified:03 Oct 2019 01:44

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