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An In Vitro Intact Globe Expansion Method for Evaluation of Cross-linking Treatments

Mattson, Matthew S. and Huynh, Joyce and Wiseman, Meredith and Coassin, Marco and Kornfield, Julia A. and Schwartz, Daniel M. (2010) An In Vitro Intact Globe Expansion Method for Evaluation of Cross-linking Treatments. Investigative Ophthalmology and Visual Science, 51 (6). pp. 3120-3128. ISSN 0146-0404. PMCID PMC2891471. doi:10.1167/iovs.09-4001.

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Purpose. To measure the tissue mechanical response to elevated intraocular pressure (IOP) using intact globe expansion of rabbit eyes. This method examined rabbit kit (2–3 weeks old) eyes as a model for weakened tissue and evaluated riboflavin/ UVA and glyceraldehyde cross-linking treatments. Methods. The ocular shape of enucleated eyes was photographed during a 24-hour period while a controlled IOP was imposed (either low IOP 22 mm Hg or high IOP 85 mm Hg). Untreated controls consisted of kit eyes tested at both low- and high IOP and adult eyes tested at high IOP. Treated kit eyes (dextran controls, riboflavin/UVA treatment of the cornea, and glyceraldehyde treatment of the entire globe) were tested at high IOP. Results. Low IOP elicited negligible creep of the sclera and very gradual creep of the cornea. In contrast, high IOP induced up to an 8% strain in the sclera and a 15% strain in the cornea of rabbit kit eyes. The expansion of adult eyes was less than one third that of kit eyes at the same, high IOP. Riboflavin/UVA treatment of corneas reduced expansion compared with that in both dextran-treated and untreated control corneas. Glyceraldehyde treatment prevented expansion of the cornea and sclera. Conclusions. The intact globe expansion method (GEM) imposes a loading geometry comparable to in vivo conditions and can quantify changes in mechanical stability as a function of testing conditions (e.g., IOP, tissue maturation, and therapeutic cross-linking) with small sample sizes and small variability. Rabbit kit eyes provide a model of weak tissue suitable for screening treatments that strengthen the cornea and sclera.

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Kornfield, Julia A.0000-0001-6746-8634
Additional Information:© 2010 Association for Research in Vision and Ophthalmology. Submitted for publication May 18, 2009; revised October 6, 2009; accepted December 15, 2009. Supported by That Man May See; National Institutes of Health Grant EY017484-01; the Jacobs Institute for Molecular Engineering for Medicine; and Caltech SURF (Summer Undergraduate Research Fellowship)
Group:Jacobs Institute for Molecular Engineering for Medicine
Funding AgencyGrant Number
Jacobs Institute for Molecular Engineering for MedicineUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
That Man May See FoundationUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC2891471
Record Number:CaltechAUTHORS:20100609-094136042
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18616
Deposited By: Tony Diaz
Deposited On:01 Jul 2010 18:49
Last Modified:08 Nov 2021 23:45

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