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Distributions of putative aerobic methanotrophs in diverse pelagic marine environments

Tavormina, Patricia L. and Ussler, William, III and Joye, Samantha B. and Harrison, Benjamin K. and Orphan, Victoria J. (2010) Distributions of putative aerobic methanotrophs in diverse pelagic marine environments. ISME Journal, 4 (5). pp. 700-710. ISSN 1751-7362. http://resolver.caltech.edu/CaltechAUTHORS:20100611-084926449

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Abstract

Aerobic methane oxidization in the pelagic ocean serves an important role in limiting methane release to the atmosphere, yet little is known about the identity and distribution of bacteria that mediate this process. The distribution of putative methane-oxidizing marine groups, OPU1, OPU3 and Group X, was assessed in different ocean provinces using a newly developed fingerprinting method (monooxygenase intergenic spacer analysis (MISA)) in combination with pmoA clone library analysis and quantitative PCR (qPCR). The distribution of these three distinct monooxygenase groups, previously reported from pelagic marine environments, was examined in 39 samples including active methane seeps in the Gulf of Mexico and Santa Monica Bay, submarine canyon heads along the California continental margin, an oligotrophic subtropical gyre and areas proximal to a hydrothermal vent in the North Fiji back-arc basin. OPU1 and OPU3 were widely and similarly distributed within the meso-and bathypelagic zone (110 to similar to 2000 m water depth) and showed a >50-fold greater abundance near methane seeps relative to non-seep sites. In contrast, Group X was predominantly recovered from samples along the California margin, at both seep and non-seep sites. All three phylotypes were below detection in the epipelagic zone to depths of 100 m. Several additional deeply branching monooxygenase sequences were also identified in this study, indicating the presence of uncharacterized groups of microorganisms potentially involved in the cycling of methane or ammonium.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/ismej.2009.155DOIArticle
http://www.nature.com/ismej/journal/v4/n5/abs/ismej2009155a.htmlPublisherArticle
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2010 International Society for Microbial Ecology. Received 3 August 2009; Revised 17 December 2009; Accepted 17 December 2009; Published online 11 February 2010. We thank Mary Lidstrom, Dan Arp and Martin Klotz for reference strains, Steve Giovannoni's group for BATS DNA, Bob Vrijenhoek for the Fiji/Lau expedition and Shana Goffredi, Olivia Mason, and 3 anonymous reviewers for assistance, critical comments, and improving the quality of this study. Support was provided by NASA (NNG06GB34G to VJO), NSF (MCB-0348492 to VJO and OCE-0085549 to SBJ, the Gordon and Betty Moore Foundation (to VJO) and the David and Lucile Packard Foundation (to WU). We thank Mary Lidstrom, Dan Arp and Martin Klotz for reference strains. Steve Giovannoni’s group for BATS DNA, Bob Vrijenhoek for the Fiji/Lau expedition and Shana Goffredi, Olivia Mason, and 3 anonymous reviewers for assistance, critical comments, and improving the quality of this study. Support was provided by NASA (NNG06GB34G to VJO), NSF (MCB-0348492 to VJO and OCE-0085549 to SBJ), the Gordon and Betty Moore Foundation (to VJO) and the David and Lucile Packard Foundation (to WU).
Funders:
Funding AgencyGrant Number
NASANNG06GB34G
NSFMCB-0348492
NSFOCE-0085549
Gordon and Betty Moore FoundationUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Subject Keywords:methane seep; methanotroph; monooxygenase; pmo
Record Number:CaltechAUTHORS:20100611-084926449
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100611-084926449
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18649
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Jul 2010 22:58
Last Modified:09 Jan 2016 03:49

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