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ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit

Jaru-Ampornpan, Peera and Shen, Kuang and Lam, Vinh Q. and Ali, Mona and Doniach, Sebastian and Jia, Tony Z. and Shan, Shu-ou (2010) ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature Structural & Molecular Biology, 17 (6). pp. 696-703. ISSN 1545-9985. PMCID PMC2917185.

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Membrane proteins impose enormous challenges to cellular protein homeostasis during their post-translational targeting, and they require chaperones to keep them soluble and translocation competent. Here we show that a novel targeting factor in the chloroplast signal recognition particle (cpSRP), cpSRP43, is a highly specific molecular chaperone that efficiently reverses the aggregation of its substrate proteins. In contrast to 'ATPases associated with various cellular activities' (AAA+) chaperones, cpSRP43 uses specific binding interactions with its substrate to mediate its 'disaggregase' activity. This disaggregase capability can allow targeting machineries to more effectively capture their protein substrates and emphasizes a close connection between protein folding and trafficking processes. Moreover, cpSRP43 provides the first example to our knowledge of an ATP-independent disaggregase and shows that efficient reversal of protein aggregation can be attained by specific binding interactions between a chaperone and its substrate.

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Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 2010 Nature Publishing Group, a division of Macmillan Publishers Limited. Received 18 March; accepted 20 April; published online 27 April 2010. We thank R. Henry (Univ. of Arkansas) for expression plasmids of cpSRP43 and LHCP, C. Robinson (Univ. of Warwick) for plasmids encoding LHCA1 and LHCB5, Z. Liu for help and advice with LHCP reconstitution, A. Sim and V. Chu of the Doniach group and D. Herschlag for help and advice with the SAXS experiments and D.C. Rees, W.M. Clemons, A. Varshavsky, N. Pierce and members of the Shan laboratory for comments on the manuscript. This work was supported by US National Institutes of Health grant GM078024 and career awards from the Burroughs Welcome Foundation, the Henry and Camille Dreyfus foundation, the Beckman foundation and the Packard foundation to S.-o.S. and by US National Institutes of Health program project grant P01-GM-66275 to S.D. and D.H. Author Contributions: P. J.-A. and S.-o.S. designed experiments; P.J.-A. and T.Z.J. performed the biochemical experiments and analyzed data; V.Q.L. and M.A. performed the SAXS experiment and analyzed data; K.S., M.A. and S.D. carried out molecular dynamics simulations of SAXS data; P. J.-A. and S.-o.S. wrote the paper.
Funding AgencyGrant Number
Burroughs Welcome FoundationUNSPECIFIED
Camille and Henry Dreyfus FoundationUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC2917185
Record Number:CaltechAUTHORS:20100623-160406505
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18783
Deposited By: Tony Diaz
Deposited On:03 Aug 2010 18:49
Last Modified:03 Oct 2019 01:47

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