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Cost of Unneeded Proteins in E. coli Is Reduced after Several Generations in Exponential Growth

Shachrai, Irit and Zaslaver, Alon and Alon, Uri and Dekel, Erez (2010) Cost of Unneeded Proteins in E. coli Is Reduced after Several Generations in Exponential Growth. Molecular Cell, 38 (5). pp. 758-767. ISSN 1097-2765. http://resolver.caltech.edu/CaltechAUTHORS:20100709-110037286

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Abstract

When E. coli cells express unneeded protein, they grow more slowly. Such penalty to fitness associated with making proteins is called protein cost. Protein cost is an important component in the cost-benefit tradeoffs that underlie the evolution of protein circuits, but its origins are still poorly understood. Here, we ask how the protein cost varies during the exponential growth phase of E. coli. We find that cells growing exponentially following an upshift from overnight culture show a large cost when producing unneeded proteins. However, after several generations, while still in exponential growth, the cells enter a phase where cost is much reduced despite vigorous unneeded protein production. We find that this reduced-cost phase depends on the ppGpp system, which adjusts the amount of ribosomes in the cell and does not occur after a downshift from rich to poor medium. These findings suggest that protein cost is a transient phenomenon that happens upon an upshift in conditions and that cost is reduced when ribosomes and other cellular systems have increased to their appropriate steady-state level in the new condition.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1016/j.molcel.2010.04.015DOIUNSPECIFIED
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSR-4YYPJPJ-1&_user=1010281&_coverDate=06%2F11%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050264&_version=1&_urlVersion=0&_userid=1010281&md5=816a6cc9babd983afd4ac9PublisherUNSPECIFIED
Additional Information:© 2010 Elsevier. Received 4 June 2009; revised 7 January 2010; accepted 2 April 2010. Published online: April 29, 2010. Available online 29 April 2010. This work was supported by the Kahn Family Foundation. Supplemental Information includes Supplemental Experimental Procedures, Supplemental References, and four figures and can be found with this article online at doi:10.1016/j.molcel.2010.04.015.
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Funding AgencyGrant Number
Kahn Family FoundationUNSPECIFIED
Subject Keywords:SYSBIO; MICROBIO; CELLCYCLE
Record Number:CaltechAUTHORS:20100709-110037286
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100709-110037286
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:18962
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:09 Jul 2010 19:49
Last Modified:26 Dec 2012 12:12

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