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Wide field-of-view microscope based on holographic focus grid illumination

Wu, Jigang and Cui, Xiquan and Zheng, Guoan and Wang, Ying Min and Lee, Lap Man and Yang, Changhuei (2010) Wide field-of-view microscope based on holographic focus grid illumination. Optics Letters, 35 (13). pp. 2188-2190. ISSN 0146-9592. https://resolver.caltech.edu/CaltechAUTHORS:20100712-153457723

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Abstract

We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6mm×5mm and acquisition time of 2.5s. The resolution is fundamentally limited by the spot size—our demonstrated average FWHM spot diameter was 0.74μm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1364/OL.35.002188DOIArticle
http://www.opticsinfobase.org/abstract.cfm?URI=ol-35-13-2188PublisherArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2010 Optical Society of America. Received March 22, 2010; revised May 19, 2010; accepted May 29, 2010; posted June 7, 2010 (Doc. ID 125762); published June 23, 2010. This work is supported by Department of Defense grant W81XWH-09-1-0051. The authors acknowledge Richard Cote and Ram Datar from the University of Miami for helpful discussions.
Funders:
Funding AgencyGrant Number
Department of DefenseW81XWH-09-1-0051
Issue or Number:13
Classification Code:OCIS codes: 170.0110, 090.2890, 170.5810
Record Number:CaltechAUTHORS:20100712-153457723
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100712-153457723
Official Citation:Jigang Wu, Xiquan Cui, Guoan Zheng, Ying Min Wang, Lap Man Lee, and Changhuei Yang, "Wide field-of-view microscope based on holographic focus grid illumination," Opt. Lett. 35, 2188-2190 (2010) http://www.opticsinfobase.org/abstract.cfm?URI=ol-35-13-2188
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:19003
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Aug 2010 17:25
Last Modified:09 Mar 2020 13:19

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