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Four-part differential leukocyte count using μflow cytometer

Shi, Wendian and Kasdan, Harvey L. and Fridge, Alan and Tai, Yu-Chong (2010) Four-part differential leukocyte count using μflow cytometer. In: MEMS 2010: 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. Proceedings: IEEE Micro Electro Mechanical Systems. IEEE , pp. 1019-1022. ISBN 978-1-4244-5764-9.

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This paper reports the four-part differential leukocyte count (DLC) of human blood using a MEMS microflow (μflow) cytometer. It is achieved with a two-color laser-induced fluorescence (LIF) detection scheme. Four types of leukocytes including neutrophils, eosinophils, lymphocytes and monocytes are identified in blood samples, which are stained by fluorescein isothiocyanate (FITC) and propidium iodide (PI). The DLC results show good correlation with the count from a commercial hematology analyzer. The whole system is also implemented into a portable instrument for space application.

Item Type:Book Section
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Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2010 IEEE. This work was supported by NSBRI under the Award Number TD01301. The authors thank all members of Caltech Micromachining Group for the helpful discussions.
Funding AgencyGrant Number
National Space Biomedical Research Institute (NSBRI)TD01301
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INSPEC Accession Number11241204
Series Name:Proceedings: IEEE Micro Electro Mechanical Systems
Record Number:CaltechAUTHORS:20100713-145241323
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:19038
Deposited By: Tony Diaz
Deposited On:14 Jul 2010 15:59
Last Modified:03 Oct 2019 01:51

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