Lac repressor purification without inactivation of DNA binding activity

Rosenberg, John M. and Khallai, Olga B. and Kopka, Mary L. and Dickerson, Richard E. and Riggs, Arthur D. (1977) Lac repressor purification without inactivation of DNA binding activity. Nucleic Acids Research, 4 (3). pp. 567-572. ISSN 0305-1048. PMCID PMC342462. doi:10.1093/nar/4.3.567. https://resolver.caltech.edu/CaltechAUTHORS:ROSnar77

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Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1093/nar/4.3.567	DOI	Article
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc342462/	PubMed Central	Article

Additional Information:

© 1977 Oxford University Press. Received November 29, 1976. This work is contribution No. 5453 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology. This work has been carried out with the support of the National Institutes of Health grants GM-12121 (RED) and HD-04420 (ADR), National Science Foundation grants PCM75-05586 (RED) and BMS75-14784 (ADR), and the Hyatt Corporation Research Fund (ADR).

Funders:

Funding Agency	Grant Number
NIH	GM-12121
NIH	HD-04420
NSF	PCM75-05586
NSF	BMS75-14784
Hyatt Corporation Research Fund	UNSPECIFIED

Other Numbering System:

Other Numbering System Name	Other Numbering System ID
Norman W. Church Laboratory of Chemical Biology	5453

Issue or Number:

PubMed Central ID:

PMC342462

DOI:

10.1093/nar/4.3.567

Record Number:

CaltechAUTHORS:ROSnar77

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:ROSnar77

Official Citation:

John M. Rosenberg, Olga B. Khallai, Mary L. Kopka, Richard E. Dickerson, Arthur D. Riggs, Lac repressor purification without inactivation of DNA binding activity, Nucleic Acids Research, Volume 4, Issue 3, 1 March 1977, Pages 567–572, https://doi.org/10.1093/nar/4.3.567

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ID Code:

1927

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

27 Feb 2006

Last Modified:

08 Nov 2021 19:43

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