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Lac repressor purification without inactivation of DNA binding activity

Rosenberg, John M. and Khallai, Olga B. and Kopka, Mary L. and Dickerson, Richard E. and Riggs, Arthur D. (1977) Lac repressor purification without inactivation of DNA binding activity. Nucleic Acids Research, 4 (3). pp. 567-572. ISSN 0305-1048. PMCID PMC342462. https://resolver.caltech.edu/CaltechAUTHORS:ROSnar77

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Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/4.3.567DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc342462/PubMed CentralArticle
Additional Information:© 1977 Oxford University Press. Received November 29, 1976. This work is contribution No. 5453 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology. This work has been carried out with the support of the National Institutes of Health grants GM-12121 (RED) and HD-04420 (ADR), National Science Foundation grants PCM75-05586 (RED) and BMS75-14784 (ADR), and the Hyatt Corporation Research Fund (ADR).
Funders:
Funding AgencyGrant Number
NIHGM-12121
NIHHD-04420
NSFPCM75-05586
NSFBMS75-14784
Hyatt Corporation Research FundUNSPECIFIED
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Norman W. Church Laboratory of Chemical Biology5453
Issue or Number:3
PubMed Central ID:PMC342462
Record Number:CaltechAUTHORS:ROSnar77
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ROSnar77
Official Citation:John M. Rosenberg, Olga B. Khallai, Mary L. Kopka, Richard E. Dickerson, Arthur D. Riggs, Lac repressor purification without inactivation of DNA binding activity, Nucleic Acids Research, Volume 4, Issue 3, 1 March 1977, Pages 567–572, https://doi.org/10.1093/nar/4.3.567
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1927
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Feb 2006
Last Modified:22 May 2020 17:10

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