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Electron Cryotomography

Tocheva, Elitza I. and Li, Zhuo and Jensen, Grant J. (2010) Electron Cryotomography. Cold Spring Harbor Perspectives in Biology, 2 (6). Art. No. a003442 . ISSN 1943-0264. PMCID PMC2869529. https://resolver.caltech.edu/CaltechAUTHORS:20100805-110641753

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Abstract

Electron cryotomography (ECT) is an emerging technology that allows thin samples such as macromolecular complexes and small bacterial cells to be imaged in 3-D in a nearly native state to “molecular” (~4 nm) resolution. As such, ECT is beginning to deliver long-awaited insight into the positions and structures of cytoskeletal filaments, cell wall elements, motility machines, chemoreceptor arrays, internal compartments, and other ultrastructures. This article describes the technique and summarizes its contributions to bacterial cell biology. For comparable recent reviews, see (Subramaniam 2005; Jensen and Briegel 2007; Murphy and Jensen 2007; Li and Jensen 2009). For reviews on the history, technical details, and broader application of electron tomography in general, see for example (Subramaniam and Milne 2004; Lucić et al. 2005; Leis et al. 2008; Midgley and Dunin-Borkowski 2009).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/cshperspect.a003442 DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869529/PubMed CentralArticle
ORCID:
AuthorORCID
Tocheva, Elitza I.0000-0002-4869-8319
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2010 Cold Spring Harbor Laboratory Press.
Issue or Number:6
PubMed Central ID:PMC2869529
Record Number:CaltechAUTHORS:20100805-110641753
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20100805-110641753
Official Citation:Cold Spring Harb Perspect Biol 2010;2:a003442
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:19294
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Aug 2010 18:47
Last Modified:03 Oct 2019 01:55

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