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Three-dimensional real-time imaging of cardiac cell motions in living embryos

Lu, Jian and Pereira, Francisco and Fraser, Scott E. and Gharib, Morteza (2008) Three-dimensional real-time imaging of cardiac cell motions in living embryos. Journal of Biomedical Optics, 13 (1). Art. No. 014006. ISSN 1083-3668.

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While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-µm fluorescent tracer particles are injected into the blood stream of 32 h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.

Item Type:Article
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URLURL TypeDescription DOIArticle
Fraser, Scott E.0000-0002-5377-0223
Gharib, Morteza0000-0002-2204-9302
Additional Information:© 2008 Society of Photo-Optical Instrumentation Engineers. Paper 06305RR received Oct. 29, 2006; revised manuscript received Aug. 31, 2007; accepted for publication Sep. 6, 2007; published online Jan. 30, 2008; corrected Jun. 13, 2008. This work was supported by NIH Grant R01RR023190.
Funding AgencyGrant Number
Subject Keywords:three-dimensional; defocusing digital particle image velocimetry; calibration; particle tracking; cardiac cell motions
Issue or Number:1
Record Number:CaltechAUTHORS:20100813-101027195
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Official Citation:Three-dimensional real-time imaging of cardiac cell motions in living embryos Jian Lu, Francisco Pereira, Scott E. Fraser, and Morteza Gharib, J. Biomed. Opt. 13, 014006 (2008), DOI:10.1117/1.2830824
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:19427
Deposited By: Tony Diaz
Deposited On:13 Aug 2010 17:25
Last Modified:03 Oct 2019 01:56

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