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An immunoglobulin promoter region is unaltered by DNA rearrangement and somatic mutation during B-cell development

Clarke, Catherine and Berenson, James and Goverman, Joan and Boyer, Paul David and Crews, Stephen and Siu, Gerald and Calame, Kathryn (1982) An immunoglobulin promoter region is unaltered by DNA rearrangement and somatic mutation during B-cell development. Nucleic Acids Research, 10 (23). pp. 7731-7749. ISSN 0305-1048.

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The V1 gene encodes the heavy chain variable region of antibodies that bind to phosphorylchollne in the Balb/c mouse. VI genes have been cloned from mouse sperm DNA, an IgM-producing tumor HPCM2 and an IgA-producing tumor M167. The transcription start site of the V1 gene has been mapped 63± 1 base pairs from the coding sequence for both α and µ transcripts. Comparison of flanking DNA sequence 574 base pairs 5' to the V1 transcription start site in sperm, HPCM2 and M167 DNA reveals that sperm and HPCM2 sequences are completely identical in this region and the M167 sequence differs from them by a single base change. Although the coding region of the V1 gene has undergone a high (4%) rate of somatic mutation in M167 we demonstrate that the somatic mutation mechanism stops near the transcription start site. These results demonstrate that initiation of V1 gene transcription remains unchanged with respect to location and 5' sequences throughout B-cell development.

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Additional Information:Copyright © 1982 Oxford University Press. Received 27 August 1982; Revised and Accepted 19 October 1982. We thank Arnold Berk, Kevin McEntee, Larry Simpson and Mark Mercola for helpful discussions concerning this work and Kevin McEntee for critically reading the manuscript. We gratefully acknowledge J. Beard for supplying avian myeloblastosis reverse transcriptase, S. Kim for supplying a subclone of M167 genomic DNA and P. Gearhart for the HPCM2 cell line. This work was supported by a grant from the National Institute of General Medical Sciences to K.C., J.G. was supported by a Gianini postdoctoral fellowship; C.C. was supported by a Genetics predoctoral training grant; P.D.B. by a Cell and Molecular Biology predoctoral training grant, S.C. & G.S. by NRSA fellowships. We are grateful to Vivian Windsor for preparation of the manuscript and to John Prehn for computer analyses of DNA sequences.
Issue or Number:23
Record Number:CaltechAUTHORS:CLAnar82
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1943
Deposited By: Tony Diaz
Deposited On:24 Feb 2006
Last Modified:02 Oct 2019 22:48

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