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Transcranial Electric Stimulation Entrains Cortical Neuronal Populations in Rats

Ozen, Simal and Sirota, Anton and Belluscio, Mariano A. and Anastassiou, Costas A. and Stark, Eran and Koch, Christof and Buzsáki, György (2010) Transcranial Electric Stimulation Entrains Cortical Neuronal Populations in Rats. Journal of Neuroscience, 30 (34). pp. 11476-11485. ISSN 0270-6474. PMCID PMC2937280.

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Low intensity electric fields have been suggested to affect the ongoing neuronal activity in vitro and in human studies. However, the physiological mechanism of how weak electrical fields affect and interact with intact brain activity is not well understood. We performed in vivo extracellular and intracellular recordings from the neocortex and hippocampus of anesthetized rats and extracellular recordings in behaving rats. Electric fields were generated by sinusoid patterns at slow frequency (0.8, 1.25 or 1.7 Hz) via electrodes placed on the surface of the skull or the dura. Transcranial electric stimulation (TES) reliably entrained neurons in widespread cortical areas, including the hippocampus. The percentage of TES phase-locked neurons increased with stimulus intensity and depended on the behavioral state of the animal. TES-induced voltage gradient, as low as 1 mV/mm at the recording sites, was sufficient to phase-bias neuronal spiking. Intracellular recordings showed that both spiking and subthreshold activity were under the combined influence of TES forced fields and network activity. We suggest that TES in chronic preparations may be used for experimental and therapeutic control of brain activity.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle CentralArticle
Anastassiou, Costas A.0000-0002-6793-0611
Koch, Christof0000-0001-6482-8067
Additional Information:© 2010 the authors. Received Oct. 22, 2009; revised July 8, 2010; accepted July 14, 2010. This work was supported by National Institutes of Health Grants NS034994 and NSF 0613414, the James M. McDonnell Foundation, the Pew Charitable Trust, the Mathers Foundation, the Gimbel Fund, the National Science Foundation, the World Class University program through the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (R31-2008-000-10008-0), and the Engineering and Physical Science Research Council. We thank Thomas Allen, Bart Krekelberg, David A. McCormick, Kenji Mizuseki, Denis Paré, and Thomas Radman for their critical input on the manuscript.
Group:Koch Laboratory (KLAB)
Funding AgencyGrant Number
NIHNSF 0613414
James M. McDonnell FoundationUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
G. Harold and Leila Y. Mathers Charitable FoundationUNSPECIFIED
Ministry of Education, Science and Technology (Korea)R31-2008-000-10008-0
Engineering and Physical Sciences Research Council (EPSRC)UNSPECIFIED
Issue or Number:34
PubMed Central ID:PMC2937280
Record Number:CaltechAUTHORS:20100913-095233059
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:19892
Deposited By: Tony Diaz
Deposited On:16 Sep 2010 21:53
Last Modified:02 Jul 2020 20:11

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