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Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells

Grohmann, Karel and Amalric, François and Crews, Stephen and Attardi, Giuseppe (1978) Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells. Nucleic Acids Research, 5 (3). pp. 637-651. ISSN 0305-1048.

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The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml camptothecin, and selected for poly(A) content by two passages through oligo(dT)-cellulose, was digested either with the nuclease P1 or with a mixture of RNases: the digestion products were then fractionated by two-dimensional electrophoresis. No "cap" structures were detected under conditions where the presence of such structures in one out of five to ten RNA molecules would have been recognized. It is, therefore, likely that "cap" structures are completely absent in HeLa cell mitochondrial poly(A)-containing RNA.

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Additional Information:Copyright © 1978 Oxford University Press. Received November 15, 1977. We wish to thank Dr. Teryl K. Frey for a generous gift of nuclease P1, and Mr. Genadie G. Sverlow and Ms. Arger Drew for valuable assistance. These investigations were supported by a research grant from the USPHS (GM-11726), an American Cancer Society (California Division) Fellowship to K.G., an EMBO Fellowship to F.A., and a USPHS Predoctoral Fellowship to S.C.
Issue or Number:3
Record Number:CaltechAUTHORS:GROnar78
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2000
Deposited By: Tony Diaz
Deposited On:01 Mar 2006
Last Modified:02 Oct 2019 22:49

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