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A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays

Liu, Mike C. and Ho, Dean and Tai, Yu-Chong (2007) A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays. In: 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE , Piscataway, NJ, pp. 993-996. ISBN 978-1-4244-0609-8. https://resolver.caltech.edu/CaltechAUTHORS:20101001-081921737

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Abstract

We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer. The combinatorial mixer is designed for screening the combinatorial effects of different compounds on cells. The monolithic fabrication method with parylene C as the basic structural material allows us to avoid wafer bonding and achieves precise alignment between microfluidic channels. As a proof-of-concept, we fabricated a device with a three-input combinatorial mixer and demonstrated that the mixer can produce all the possible combinations. Also, we demonstrated the ability to culture cells on-chip and performed a simple cell assay on-chip using trypan blue to stain dead cells.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1109/NEMS.2007.352185 DOIArticle
http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=4160488PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2007 IEEE.
Subject Keywords:3-d microfluidic; cell culture; parylene C
Record Number:CaltechAUTHORS:20101001-081921737
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101001-081921737
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20241
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Oct 2010 22:24
Last Modified:03 Oct 2019 02:07

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