Liu, Mike C. and Ho, Dean and Tai, Yu-Chong (2007) A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays. In: 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE , Piscataway, NJ, pp. 993-996. ISBN 978-1-4244-0609-8. https://resolver.caltech.edu/CaltechAUTHORS:20101001-081921737
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Abstract
We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer. The combinatorial mixer is designed for screening the combinatorial effects of different compounds on cells. The monolithic fabrication method with parylene C as the basic structural material allows us to avoid wafer bonding and achieves precise alignment between microfluidic channels. As a proof-of-concept, we fabricated a device with a three-input combinatorial mixer and demonstrated that the mixer can produce all the possible combinations. Also, we demonstrated the ability to culture cells on-chip and performed a simple cell assay on-chip using trypan blue to stain dead cells.
Item Type: | Book Section | |||||||||
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Additional Information: | © 2007 IEEE. | |||||||||
Subject Keywords: | 3-d microfluidic; cell culture; parylene C | |||||||||
DOI: | 10.1109/NEMS.2007.352185 | |||||||||
Record Number: | CaltechAUTHORS:20101001-081921737 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20101001-081921737 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 20241 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Tony Diaz | |||||||||
Deposited On: | 01 Oct 2010 22:24 | |||||||||
Last Modified: | 08 Nov 2021 23:58 |
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