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Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA

Grossman, Lawrence I. and Parker, Richard C. and Watson, Robert M. and Chandler, Sarah E. W. and Teplitz, Marlyn (1977) Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA. Nucleic Acids Research, 4 (5). pp. 1267-1271. ISSN 0305-1048. PMCID PMC343754. https://resolver.caltech.edu/CaltechAUTHORS:GROnar77

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Abstract

A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/4.5.1267DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc343754/PubMed CentralArticle
Additional Information:© 1977 Oxford University Press. Dedicated to the memory of Professor Jerome Vinograd. Received February 10, 1977. We are grateful to Mrs. Paula Berry, City of Hope National Medical Center, for help with the karyotype preparations. This work was supported by grants GM 21704 from NIH at Wayne State University and GM 15327 and CA 08014 from NIH at Caltech. This is contribution No. 5501 from the Division of Chemistry and Chemical Engineering.
Funders:
Funding AgencyGrant Number
NIHGM 21704
NIHGM 15327-09
NIHCA 08014
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Caltech Division of Chemistry and Chemical Engineering5501
Issue or Number:5
PubMed Central ID:PMC343754
Record Number:CaltechAUTHORS:GROnar77
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GROnar77
Official Citation:Lawrence I. Grossman, Richard C. Parker, Robert M. Watson, Sarah E.W. Chandler, Marlyn Teplitz, Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA , Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1267–1272, https://doi.org/10.1093/nar/4.5.1267
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2026
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Mar 2006
Last Modified:22 May 2020 17:22

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