Grossman, Lawrence I. and Parker, Richard C. and Watson, Robert M. and Chandler, Sarah E. W. and Teplitz, Marlyn (1977) Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA. Nucleic Acids Research, 4 (5). pp. 1267-1271. ISSN 0305-1048. PMCID PMC343754. doi:10.1093/nar/4.5.1267. https://resolver.caltech.edu/CaltechAUTHORS:GROnar77
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Abstract
A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.
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| Additional Information: | © 1977 Oxford University Press. Dedicated to the memory of Professor Jerome Vinograd. Received February 10, 1977. We are grateful to Mrs. Paula Berry, City of Hope National Medical Center, for help with the karyotype preparations. This work was supported by grants GM 21704 from NIH at Wayne State University and GM 15327 and CA 08014 from NIH at Caltech. This is contribution No. 5501 from the Division of Chemistry and Chemical Engineering. | |||||||||
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| Issue or Number: | 5 | |||||||||
| PubMed Central ID: | PMC343754 | |||||||||
| DOI: | 10.1093/nar/4.5.1267 | |||||||||
| Record Number: | CaltechAUTHORS:GROnar77 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:GROnar77 | |||||||||
| Official Citation: | Lawrence I. Grossman, Richard C. Parker, Robert M. Watson, Sarah E.W. Chandler, Marlyn Teplitz, Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA , Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1267–1272, https://doi.org/10.1093/nar/4.5.1267 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 2026 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 02 Mar 2006 | |||||||||
| Last Modified: | 08 Nov 2021 19:44 |
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