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Sub-pixel resolving optofluidic microscope for on-chip cell imaging

Zheng, Guoan and Lee, Seung Ah and Yang, Samuel and Yang, Changhuei (2010) Sub-pixel resolving optofluidic microscope for on-chip cell imaging. Lab on a Chip, 2010 (10 (22). pp. 3125-3129. ISSN 1473-0197.

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We report the implementation of a fully on-chip, lensless, sub-pixel resolving optofluidic microscope (SROFM). The device utilizes microfluidic flow to deliver specimens directly across a complementary metal oxide semiconductor (CMOS) sensor to generate a sequence of low-resolution (LR) projection images, where resolution is limited by the sensor's pixel size. This image sequence is then processed with a pixel super-resolution algorithm to reconstruct a single high resolution (HR) image, where features beyond the Nyquist rate of the LR images are resolved. We demonstrate the device's capabilities by imaging microspheres, protist Euglena gracilis, and Entamoeba invadens cysts with sub-cellular resolution and establish that our prototype has a resolution limit of 0.75 microns. Furthermore, we also apply the same pixel super-resolution algorithm to reconstruct HR videos in which the dynamic interaction between the fluid and the sample, including the in-plane and out-of-plane rotation of the sample within the flow, can be monitored in high resolution. We believe that the powerful combination of both the pixel super-resolution and optofluidic microscopy techniques within our SROFM is a significant step forwards toward a simple, cost-effective, high throughput and highly compact imaging solution for biomedical and bioscience needs.

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URLURL TypeDescription Information
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2010 The Royal Society of Chemistry. Received 20th July 2010, Accepted 24th August 2010. First published on the web 29th September 2010. We are grateful for the constructive discussions with and generous help from Dr Jigang Wu, Mr. Lap Man Lee, Mr. Jian Ren and Mr. Shuo Pang from Caltech. We thank Dr Daniel Echinger from New York University Medical Center for sending us Entamoeba invadens cyst samples. We acknowledge funding support from the Coulter Foundation and Gates Foundation.
Funding AgencyGrant Number
Wallace Coulter FoundationUNSPECIFIED
Bill and Melinda Gates FoundationUNSPECIFIED
Issue or Number:10 (22
Record Number:CaltechAUTHORS:20101004-084011087
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Official Citation:Zheng, G., S. A. Lee, et al. (2010). "Sub-pixel resolving optofluidic microscope for on-chip cell imaging." Lab on a Chip 10(22): 3125-3129. DOI:10.1039/c0lc00213e
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20266
Deposited By: Tony Diaz
Deposited On:16 Nov 2010 19:13
Last Modified:09 Mar 2020 13:19

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