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A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood

Wang, Jun and Ahmad, Habib and Ma, Chao and Shi, Qihui and Vermesh, Ophir and Vermesh, Udi and Heath, James (2010) A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood. Lab on a Chip, 2010 (10 (22). pp. 3157-3162. ISSN 1473-0197. PMCID PMC3651856. https://resolver.caltech.edu/CaltechAUTHORS:20101012-100925718

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Abstract

We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody “barcode” arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.


Item Type:Article
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http://dx.doi.org/10.1039/C0LC00132EDOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2010/LC/c0lc00132ePublisherArticle
http://www.rsc.org/suppdata/LC/C0/C0LC00132E/C0LC00132E.PDFPublisherSupporting Information
http://www.rsc.org/suppdata/LC/C0/C0LC00132E/SIVIDEO1.WMVPublisherSupporting Information
http://www.rsc.org/suppdata/LC/C0/C0LC00132E/SIVIDEO2.WMVPublisherSupporting Information
http://www.rsc.org/suppdata/LC/C0/C0LC00132E/SIVIDEO3.WMVPublisherSupporting Information
http://www.rsc.org/suppdata/LC/C0/C0LC00132E/SIVIDEO4.WMVPublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651856/PubMed CentralArticle
ORCID:
AuthorORCID
Heath, James0000-0001-5356-4385
Additional Information:© 2010 Royal Society of Chemistry. Received 19th June 2010; Accepted 9th September 2010. First published on the web 6th October 2010. This work was supported by the National Cancer Institute Grant No. 5U54 CA119347 (J.R.H. and P.I.), by a gift from the Jean Perkins Foundation and by the Ben & Catherine Ivy Foundation.
Funders:
Funding AgencyGrant Number
National Cancer Institute5U54 CA119347
Jean Perkins FoundationUNSPECIFIED
Ben and Catherine Ivy FoundationUNSPECIFIED
Issue or Number:10 (22
PubMed Central ID:PMC3651856
Record Number:CaltechAUTHORS:20101012-100925718
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101012-100925718
Official Citation:Wang, J., H. Ahmad, et al. (2010). "A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood." Lab on a Chip 10(22): 3157-3162. DOI: 10.1039/C0LC00132E
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20396
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:16 Nov 2010 19:22
Last Modified:03 Oct 2019 02:09

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