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Histone Demethylase JmjD2A Regulates Neural Crest Specification

Strobl-Mazzulla, Pablo Hernan and Sauka-Spengler, Tatjana and Bronner-Fraser, Marianne (2010) Histone Demethylase JmjD2A Regulates Neural Crest Specification. Developmental Cell, 19 (3). pp. 460-468. ISSN 1534-5807. PMCID PMC2939072. https://resolver.caltech.edu/CaltechAUTHORS:20101027-150825589

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Abstract

The neural crest is a multipotent stem cell-like population that is induced during gastrulation, but only acquires its characteristic morphology, migratory ability, and gene expression profile after neurulation. This raises the intriguing possibility that precursors are actively maintained by epigenetic influences in a stem cell-like state. Accordingly, we report that dynamic histone modifications are critical for proper temporal control of neural crest gene expression in vivo. The histone demethylase, JumonjiD2A (JmjD2A/KDM4A), is expressed in the forming neural folds. Loss of JmjD2A function causes dramatic downregulation of neural crest specifier genes analyzed by multiplex NanoString and in situ hybridization. Importantly, in vivo chromatin immunoprecipitation reveals direct stage-specific interactions of JmjD2A with regulatory regions of neural crest genes, and associated temporal modifications in methylation states of lysine residues directly affected by JmjD2A activity. Our findings show that chromatin modifications directly control neural crest genes in vertebrate embryos via modulating histone methylation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.devcel.2010.08.009DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939072/PubMed CentralArticle
ORCID:
AuthorORCID
Strobl-Mazzulla, Pablo Hernan0000-0003-0591-6168
Sauka-Spengler, Tatjana0000-0001-9289-0263
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2010 Elsevier. Received 22 September 2009; revised 21 June 2010; accepted 13 August 2010. Published: September 13, 2010. Available online 14 September 2010. We thank Dr. Scott Fraser for critical reading of the manuscript. This work was supported by USPHS grant HD037105.
Funders:
Funding AgencyGrant Number
NIHHD037105
Issue or Number:3
PubMed Central ID:PMC2939072
Record Number:CaltechAUTHORS:20101027-150825589
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101027-150825589
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20568
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Nov 2010 18:12
Last Modified:09 Mar 2020 13:19

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