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Alpha-conotoxin ImI disrupts central control of swimming in the medicinal leech

Wagenaar, Daniel A. and Gonzalez, Ruben and Ries, David C. and Kristan, Wiliam B., Jr. and French, Kathleen A. (2010) Alpha-conotoxin ImI disrupts central control of swimming in the medicinal leech. Neuroscience Letters, 485 (3). pp. 151-156. ISSN 0304-3940. PMCID PMC2956871. http://resolver.caltech.edu/CaltechAUTHORS:20101028-094759611

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Abstract

Medicinal leeches (Hirudo spp.) swim using a metachronal, front-to-back undulation. The behavior is generated by central pattern generators (CPGs) distributed along the animal’s midbody ganglia and is coordinated by both central and peripheral mechanisms. Here we report that a component of the venom of Conus imperialis, α-conotoxin ImI, known to block nicotinic acetyl-choline receptors in other species, disrupts swimming. Leeches injected with the toxin swam in circles with exaggerated dorsoventral bends and reduced forward velocity. Fictive swimming in isolated nerve cords was even more strongly disrupted, indicating that the toxin targets the CPGs and central coordination, while peripheral coordination partially rescues the behavior in intact animals.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.neulet.2010.08.078DOIArticle
http://www.sciencedirect.com/science/article/pii/S0304394010011584PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956871/PubMed CentralArticle
ORCID:
AuthorORCID
Wagenaar, Daniel A.0000-0002-6222-761X
Additional Information:© 2010 Elsevier Ireland Ltd. Received 26 May 2010; revised 5 August 2010; accepted 26 August 2010. Available online 15 September 2010. We thank Dr. Baldomero Olivera and his laboratory for supplying Conus venoms and ImI in the early phases of this work. This work was supported by NIH Research Grants MH43396 and NS35336 (to WBK), by a Senior Research Fellowship from the Broad Foundations (to DAW) and by Microsoft Research Labs. DAW holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.
Funders:
Funding AgencyGrant Number
NIHMH43396
NIHNS35336
Eli and Edythe Broad FoundationUNSPECIFIED
Microsoft Research LabsUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Subject Keywords:Swimming; Central pattern generator; Intersegmental coordination; Medicinal leech; Hirudo verbana
PubMed Central ID:PMC2956871
Record Number:CaltechAUTHORS:20101028-094759611
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20101028-094759611
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20580
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:28 Oct 2010 20:37
Last Modified:20 Nov 2015 00:17

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