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Portable Filter-Based Microdevice for Detection and Characterization of Circulating Tumor Cells

Lin, Henry K. and Zheng, Siyang and Williams, Anthony J. and Balic, Marija and Groshen, Susan and Scher, Howard I. and Fleisher, Martin and Stadler, Walter and Datar, Ram H. and Tai, Yu-Chong and Cote, Richard J. (2010) Portable Filter-Based Microdevice for Detection and Characterization of Circulating Tumor Cells. Clinical Cancer Research, 16 (20). pp. 5011-5018. ISSN 1078-0432. http://resolver.caltech.edu/CaltechAUTHORS:20101103-090849041

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Abstract

Purpose: Sensitive detection and characterization of circulating tumor cells (CTC) could revolutionize the approach to patients with early-stage and metastatic cancer. The current methodologies have significant limitations, including limited capture efficiency and ability to characterize captured cells. Here, we report the development of a novel parylene membrane filter-based portable microdevice for size-based isolation with high recovery rate and direct on-chip characterization of captured CTC from human peripheral blood. Experimental Design: We evaluated the sensitivity and efficiency of CTC capture in a model system using blood samples from healthy donors spiked with tumor cell lines. Fifty-nine model system samples were tested to determine the recovery rate of the microdevice. Moreover, 10 model system samples and 57 blood samples from cancer patients were subjected to both membrane microfilter device and CellSearch platform enumeration for direct comparison. Results: Using the model system, the microdevice achieved >90% recovery with probability of 95% recovering at least one cell when five are seeded in 7.5 mL of blood. CTCs were identified in 51 of 57 patients using the microdevice, compared with only 26 patients with the CellSearch method. When CTCs were detected by both methods, greater numbers were recovered by the microfilter device in all but five patients. Conclusions: This filter-based microdevice is both a capture and analysis platform, capable of multiplexed imaging and genetic analysis. The microdevice presented here has the potential to enable routine CTC analysis in the clinical setting for the effective management of cancer patients.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1158/1078-0432.CCR-10-1105 DOIUNSPECIFIED
http://clincancerres.aacrjournals.org/content/16/20/5011.abstractPublisherUNSPECIFIED
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2010 American Association for Cancer Research. Received April 29, 2010. Revision received July 8, 2010. Accepted July 12, 2010. H.K. Lin and S. Zheng contributed equally. H.K. Lin, S. Zheng, and A.J. Williams performed the experiments. All authors contributed to experimental design, data analysis, and manuscript writing. We thank the participating patients for the source of clinical blood samples. Grant Support: National Institutes of Health Grant 1R21CA123027 (RJC), Doheny Eye Institute Specialized Imaging Core grant EY03040. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIH1R21CA123027
Doheny Eye Institute EY03040
Record Number:CaltechAUTHORS:20101103-090849041
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20101103-090849041
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20646
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Nov 2010 22:34
Last Modified:16 May 2017 22:58

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