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Modeling the kinetics of hybridization in microarrays

Vikalo, H. and Hassibi, B. and Stojnic, M. and Hassibi, A. (2007) Modeling the kinetics of hybridization in microarrays. In: 2007 IEEE International Workshop on Genomic Signal Processing and Statistics. IEEE , pp. 1-4. ISBN 978-1-4244-0998-3.

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Conventional fluorescent-based microarrays acquire data after the hybridization phase. In this phase the targets analytes (i.e., DNA fragments) bind to the capturing probes on the array and supposedly reach a steady state. Accordingly, microarray experiments essentially provide only a single, steady-state data point of the hybridization process. On the other hand, a novel technique (i.e., realtime microarrays) capable of recording the kinetics of hybridization in fluorescent-based microarrays has recently been proposed in [5]. The richness of the information obtained therein promises higher signal-to-noise ratio, smaller estimation error, and broader assay detection dynamic range compared to the conventional microarrays. In the current paper, we develop a probabilistic model of the kinetics of hybridization and describe a procedure for the estimation of its parameters which include the binding rate and target concentration. This probabilistic model is an important step towards developing optimal detection algorithms for the microarrays which measure the kinetics of hybridization, and to understanding their fundamental limitations.

Item Type:Book Section
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Additional Information:© 2007 IEEE. Issue Date: 10-12 June 2007. Date of Current Version: 29 October 2007.
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INSPEC Accession Number9776801
Record Number:CaltechAUTHORS:20101104-111636482
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20672
Deposited By: Ruth Sustaita
Deposited On:04 Nov 2010 20:13
Last Modified:09 Nov 2021 00:02

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