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Modeling the kinetics of hybridization in microarrays

Vikalo, H. and Hassibi, B. and Stojnic, M. and Hassibi, A. (2007) Modeling the kinetics of hybridization in microarrays. In: 2007 IEEE International Workshop on Genomic Signal Processing and Statistics. IEEE , pp. 1-4. ISBN 978-1-4244-0998-3. https://resolver.caltech.edu/CaltechAUTHORS:20101104-111636482

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Abstract

Conventional fluorescent-based microarrays acquire data after the hybridization phase. In this phase the targets analytes (i.e., DNA fragments) bind to the capturing probes on the array and supposedly reach a steady state. Accordingly, microarray experiments essentially provide only a single, steady-state data point of the hybridization process. On the other hand, a novel technique (i.e., realtime microarrays) capable of recording the kinetics of hybridization in fluorescent-based microarrays has recently been proposed in [5]. The richness of the information obtained therein promises higher signal-to-noise ratio, smaller estimation error, and broader assay detection dynamic range compared to the conventional microarrays. In the current paper, we develop a probabilistic model of the kinetics of hybridization and describe a procedure for the estimation of its parameters which include the binding rate and target concentration. This probabilistic model is an important step towards developing optimal detection algorithms for the microarrays which measure the kinetics of hybridization, and to understanding their fundamental limitations.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1109/GENSIPS.2007.4365828DOIUNSPECIFIED
http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=4365828&tag=1PublisherUNSPECIFIED
Additional Information:© 2007 IEEE. Issue Date: 10-12 June 2007. Date of Current Version: 29 October 2007.
Other Numbering System:
Other Numbering System NameOther Numbering System ID
INSPEC Accession Number9776801
DOI:10.1109/GENSIPS.2007.4365828
Record Number:CaltechAUTHORS:20101104-111636482
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101104-111636482
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20672
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Nov 2010 20:13
Last Modified:09 Nov 2021 00:02

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