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Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells

Shin, Young Shik and Ahmad, Habib and Shi, Qihui and Kim, Hyungjun and Pascal, Tod A. and Fan, Rong and Goddard, William A., III and Heath, James R. (2010) Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. ChemPhysChem, 11 (14). pp. 3063-3069. ISSN 1439-4235. PMCID PMC3681607. https://resolver.caltech.edu/CaltechAUTHORS:20101108-150340183

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Abstract

The optimization of chemistries to enable the patterning of miniaturized DNA barcodes using microfluidics flow channels is described (see picture). Experiment and theory reveal that solvent mixtures in which counterions are strongly associated with the negatively charged DNA oligomers may be harnessed to produce high quality, high density DNA microarray patterns over a large area.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/cphc.201000528 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/cphc.201000528/abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681607/PubMed CentralArticle
ORCID:
AuthorORCID
Pascal, Tod A.0000-0003-2096-1143
Goddard, William A., III0000-0003-0097-5716
Heath, James R.0000-0001-5356-4385
Additional Information:© 2010 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim. Received: July 1, 2010. Published online on August 16, 2010. This work was supported by National Cancer Institute Grant No. 5U54 CA119347 (J.R.H., P.I.) and by a gift from the Jean Perkins Foundation. H. K, T. A. P and W. A. G also acknowledge support from the WCU programs through NRF of Korea funded by the MEST (R31–2008–000–10055–0).
Funders:
Funding AgencyGrant Number
NIH5U54 CA119347
National Research Foundation of KoreaR31-2008-000-10055-0
Jean Perkins FoundationUNSPECIFIED
National Cancer InstituteUNSPECIFIED
Subject Keywords:microarrays; microfluidics; proteins; single-cell studies; surface chemistry
Issue or Number:14
PubMed Central ID:PMC3681607
Record Number:CaltechAUTHORS:20101108-150340183
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101108-150340183
Official Citation:Shin, Y. S., Ahmad, H., Shi, Q., Kim, H., Pascal, T. A., Fan, R., Goddard, W. A. and Heath, J. R. (2010), Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. ChemPhysChem, 11: 3063–3069. doi: 10.1002/cphc.201000528
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:20721
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Nov 2010 18:18
Last Modified:03 Oct 2019 02:14

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