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Alignment between PIN1 Polarity and Microtubule Orientation in the Shoot Apical Meristem Reveals a Tight Coupling between Morphogenesis and Auxin Transport

Heisler, Marcus G. B. and Hamant, Olivier and Krupinski, Pawel and Uyttewaal, Magalie and Ohno, Carolyn and Jönsson, Henrik and Traas, Jan and Meyerowitz, Elliot M. (2010) Alignment between PIN1 Polarity and Microtubule Orientation in the Shoot Apical Meristem Reveals a Tight Coupling between Morphogenesis and Auxin Transport. PLoS Biology, 8 (10). Art. No. e1000516 . ISSN 1544-9173. PMCID PMC2957402. http://resolver.caltech.edu/CaltechAUTHORS:20101122-095339674

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Abstract

Morphogenesis during multicellular development is regulated by intercellular signaling molecules as well as by the mechanical properties of individual cells. In particular, normal patterns of organogenesis in plants require coordination between growth direction and growth magnitude. How this is achieved remains unclear. Here we show that in Arabidopsis thaliana, auxin patterning and cellular growth are linked through a correlated pattern of auxin efflux carrier localization and cortical microtubule orientation. Our experiments reveal that both PIN1 localization and microtubule array orientation are likely to respond to a shared upstream regulator that appears to be biomechanical in nature. Lastly, through mathematical modeling we show that such a biophysical coupling could mediate the feedback loop between auxin and its transport that underlies plant phyllotaxis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pbio.1000516 DOIArticle
http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000516PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957402/PubMed CentralArticle
ORCID:
AuthorORCID
Heisler, Marcus G. B.0000-0001-5644-8398
Jönsson, Henrik0000-0003-2340-588X
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2010 Heisler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received May 13, 2010; Accepted September 1, 2010; Published October 19, 2010. Academic Editor: Ottoline Leyser, University of York, United Kingdom. Funding: This work is supported by the International Human Frontier Science Program Organization, by United States Department of Energy grant FG02-88ER13873 (to EMM), and by the Swedish Research Council (to HJ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank Alexandre Cunha for help in identifying important references, as well as IFR128 Platim for help with imaging. Author Contributions: The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: MGH OH PK HJ JT EMM. Performed the experiments: MGH OH PK MU CO. Analyzed the data: MGH OH PK MU HJ JT EMM. Contributed reagents/materials/analysis tools: MGH OH PK CO. Wrote the paper: MGH OH PK HJ JT EMM.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
Department of Energy (DOE)DE-FG02-88ER13873
Swedish Research CouncilUNSPECIFIED
PubMed Central ID:PMC2957402
Record Number:CaltechAUTHORS:20101122-095339674
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20101122-095339674
Official Citation:Heisler MG, Hamant O, Krupinski P, Uyttewaal M, Ohno C, Jönsson H, et al. (2010) Alignment between PIN1 Polarity and Microtubule Orientation in the Shoot Apical Meristem Reveals a Tight Coupling between Morphogenesis and Auxin Transport. PLoS Biol 8(10): e1000516. doi:10.1371/journal.pbio.1000516
Usage Policy:This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ID Code:20937
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Nov 2010 00:11
Last Modified:19 Sep 2017 17:12

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