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Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish

Quesada-Hernández, Elena and Caneparo, Luca and Schneider, Sylvia and Winkler, Sylke and Liebling, Michael and Fraser, Scott E. and Heisenberg, Carl-Philipp (2010) Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish. Current Biology, 20 (21). pp. 1966-1972. ISSN 0960-9822. http://resolver.caltech.edu/CaltechAUTHORS:20101206-141601196

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Abstract

The development of multicellular organisms is dependent on the tight coordination between tissue growth and morphogenesis. The stereotypical orientation of cell divisions has been proposed to be a fundamental mechanism by which proliferating and growing tissues take shape. However, the actual contribution of stereotypical division orientation (SDO) to tissue morphogenesis is unclear. In zebrafish, cell divisions with stereotypical orientation have been implicated in both body-axis elongation and neural rod formation [[1] and [2]], although there is little direct evidence for a critical function of SDO in either of these processes. Here we show that SDO is required for formation of the neural rod midline during neurulation but dispensable for elongation of the body axis during gastrulation. Our data indicate that SDO during both gastrulation and neurulation is dependent on the noncanonical Wnt receptor Frizzled 7 (Fz7) and that interfering with cell division orientation leads to severe defects in neural rod midline formation but not body-axis elongation. These findings suggest a novel function for Fz7-controlled cell division orientation in neural rod midline formation during neurulation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2010.10.009 DOIArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5739-4026
Additional Information:© 2010 Elsevier Ltd. Received 13 July 2010; revised 3 September 2010; accepted 4 October 2010. Published online: October 21, 2010. Available online 21 October 2010. We are grateful to Jon Clarke, Andy Oates, and Garrett Greenan for reading earlier versions of this manuscript. We thank J. Peychl, H. Ibarra, and P. Pitrone for excellent assistance and advice in multi-photon microscopy and D. White for assistance during the image-processing steps. We also thank D. Panhans for technical assistance, the whole Heisenberg laboratory for useful comments and discussions, and E. Lehmann, J. Hückmann, and G. Junghans for excellent fish care. This work was supported by grants from the Fundación Caja Madrid to E.Q.H. and the Institute of Science and Technology Austria, the Max-Planck-Society, and the Deutsche Forschungsgemeinschaft to C.P.H.
Funders:
Funding AgencyGrant Number
Fundación Caja MadridUNSPECIFIED
Institute of Science and Technology AustriaUNSPECIFIED
Max-Planck-SocietyUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Record Number:CaltechAUTHORS:20101206-141601196
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20101206-141601196
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21193
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Dec 2010 00:54
Last Modified:01 Jan 2016 02:55

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