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Yielding Behavior in Injectable Hydrogels from Telechelic Proteins

Olsen, Bradley D. and Kornfield, Julia A. and Tirrell, David A. (2010) Yielding Behavior in Injectable Hydrogels from Telechelic Proteins. Macromolecules, 43 (21). pp. 9094-9099. ISSN 0024-9297. PMCID PMC3017468. https://resolver.caltech.edu/CaltechAUTHORS:20101207-081608961

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Abstract

Injectable hydrogels show substantial promise for use in minimally invasive tissue engineering and drug delivery procedures. A new injectable hydrogel material, developed from recombinant telechelic proteins expressed in E. coli, demonstrates shear thinning by 3 orders of magnitude at large strains. Large-amplitude oscillatory shear illustrates that shear thinning is due to yielding within the bulk of the gel, and the rheological response and flow profiles are consistent with a shear-banding mechanism for yielding. The sharp yielding transition and large magnitude of the apparent shear thinning allow gels to be injected through narrow gauge needles with only gentle hand pressure. After injection the gels reset to full elastic strength in seconds due to rapid re-formation of the physical network junctions, allowing self-supporting structures to be formed. The shear thinning and recovery behavior is largely independent of the midblock length, enabling genetic engineering to be used to control the equilibrium modulus of the gel without loss of the characteristic yielding behavior. The shear-banding mechanism localizes deformation during flow into narrow regions of the gels, allowing more than 95% of seeded cells to survive the injection process.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ma101434a DOIArticle
http://pubs.acs.org/doi/full/10.1021/ma101434aPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017468PubMed CentralArticle
ORCID:
AuthorORCID
Kornfield, Julia A.0000-0001-6746-8634
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2010 American Chemical Society. Received June 28, 2010; Revised Manuscript Received September 27, 2010. Published on Web 10/13/2010. This work was supported by the NSF Center for the Science and Engineering of Materials and NIH Grant EB1971. B.D.O. was supported by Award F32GM0834 from the National Institute of General Medical Sciences and by a Beckman Institute Postdoctoral Fellowship. We thank Professor S. E. Fraser for suggesting the method of flow visualization reported in Figure 3.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
NIHEB1971
NIH Postdoctoral FellowshipF32GM0834
Caltech Beckman InstituteUNSPECIFIED
Issue or Number:21
PubMed Central ID:PMC3017468
Record Number:CaltechAUTHORS:20101207-081608961
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101207-081608961
Official Citation:Yielding Behavior in Injectable Hydrogels from Telechelic Proteins Bradley D. Olsen, Julia A. Kornfield, David A. Tirrell Macromolecules 2010 43 (21), 9094-9099
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21204
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Dec 2010 00:26
Last Modified:03 Oct 2019 02:21

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