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Fully automated, sequential tilt-series acquisition with Leginon

Suloway, Christian and Shi, Jian and Cheng, Anchi and Pulokas, James and Carragher, Bridget and Potter, Clinton S. and Zheng, Shawn Q. and Agard, David A. and Jensen, Grant J. (2009) Fully automated, sequential tilt-series acquisition with Leginon. Journal of Structural Biology, 167 (1). pp. 11-18. ISSN 1047-8477. PMCID PMC2724967. http://resolver.caltech.edu/CaltechAUTHORS:20101209-110311836

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Abstract

Electron tomography has become a uniquely powerful tool for investigating the structures of individual cells, viruses, and macromolecules. Data collection is, however, time consuming and requires expensive instruments. To optimize productivity, we have incorporated one of the existing tilt-series acquisition programs, UCSF Tomo, into the well-developed automatic electron microscopy data collection package Leginon to enable fully automatic, sequential tilt-series acquisition. Here we describe how UCSF Tomo was integrated into Leginon, what users must do to set up a data collection session, how the automatic collection proceeds, how archived data about the process can be accessed and used, and how the software has been tested.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jsb.2009.03.019 DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724967/PubMed CentralArticle
ORCID:
AuthorORCID
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2009 Elsevier Inc. Received 21 October 2008; revised 27 March 2009; accepted 31 March 2009. Available online 8 April 2009. We thank Drs. Bill Tivol, Prabha Dias, Jane Ding, Lu Gan, Ariane Briegel, Elizabeth Wright, Zhou Li and Alasdair McDowall for advice and testing during the development of MSI Tomography. This work was supported in part by NIH Grants R01 AI067548 and P50 GM082545 and DOE Grant DE-FG02-04ER63785 to GJJ, a Searle Scholar Award to GJJ, the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute. Leginon development at the National Resource for Automated Molecular Microscopy is supported by the National Institutes of Health though the National Center for Research Resources’ P41 program (RR17573 and R01 RR023093).
Funders:
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 GM082545
Department of Energy (DOE)DE-FG02-04ER63785
Searle Scholars ProgramUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
NIHRR17573
NIHR01 RR023093
Subject Keywords:TEM; Cryo-electron microscopy; Electron microscopy; Automation; Tomography
PubMed Central ID:PMC2724967
Record Number:CaltechAUTHORS:20101209-110311836
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20101209-110311836
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21267
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Dec 2010 21:00
Last Modified:14 Dec 2015 18:30

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