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Combined chemical and genetic approach to inhibit proteolysis by the proteasome

Collins, Galen A. and Gomez, Tara Adele and Deshaies, Raymond J. and Tansey, William P. (2010) Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast, 27 (11). pp. 965-974. ISSN 0749-503X. PMCID PMC3566228.

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Regulated protein destruction by the proteasome is crucial for the maintenance of normal cellular homeostasis. Much of our understanding of proteasome function stems from the use of drugs that inhibit its activity. Curiously, despite the importance of proteasomal proteolysis, previous studies have found that proliferation of the yeast Saccharomyces cerevisiae is relatively resistant to the effects of proteasome inhibitors such as MG132, even in the presence of mutations that increase inhibitor levels in cells. We reasoned that part of the resistance of S. cerevisiae to proteasome inhibitors stems from the fact that most proteasome inhibitors preferentially target the chymotryptic activity of the proteasome, and that the caspase-like and tryptic sites within the 20S core could compensate for proteasome function under these conditions. To test this hypothesis, we generated a strain of yeast in which the gene encoding the drug efflux pump Pdr5 is deleted, and the tryptic and caspase-like proteasome activities are inactivated by mutation. We find that this strain has dramatically increased sensitivity to the proteasome inhibitor MG132. Under these conditions, treatment of yeast with MG132 blocks progression through the cell cycle, increases the accumulation of polyubiquitylated proteins and decreases the ability to induce transcription of certain genes. These results highlight the contribution of the caspase-like and tryptic activities of the proteasome to its function, and provide a strategy to potently block proteasomal proteolysis in yeast that has practical applications.

Item Type:Article
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URLURL TypeDescription CentralArticle
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2010 John Wiley & Sons, Ltd. Received: 9 January 2010; Accepted: 30 May 2010; Article first published online: 2 Nov. 2010. For reagents, we thank S. Berger, B. Laurent, M. Hochstrasser, Proteolix Inc. and Millenium Pharmaceuticals. We thank P. Moody for technical assistance, S. Mont for sharing unpublished data and A. Daulny, F. Geng and A. Leung for valuable comments. G.A.C. was supported by the Watson School of Biological Sciences. R.J.D. is an investigator of the HHMI. T.A.G. was supported by an NSF pre-doctoral fellowship. These studies are supported by NIH grant GM067728 to W.P.T.
Funding AgencyGrant Number
Watson School of Biological SciencesUNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:proteasome; inhibitor; cell-cycle; proteolysis; transcription
Issue or Number:11
PubMed Central ID:PMC3566228
Record Number:CaltechAUTHORS:20101210-081023629
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Official Citation:Collins, G. A., Gomez, T. A., Deshaies, R. J. and Tansey, W. P. (2010), Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast, 27: 965–974. doi: 10.1002/yea.1805
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21288
Deposited By: Tony Diaz
Deposited On:11 Dec 2010 00:24
Last Modified:03 Oct 2019 02:22

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