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Altering Glypican-1 levels modulates canonical Wnt signaling during trigeminal placode development

Shiau, Celia E. and Hua, Na and Bronner-Fraser, Marianne (2010) Altering Glypican-1 levels modulates canonical Wnt signaling during trigeminal placode development. Developmental Biology, 348 (1). pp. 107-118. ISSN 0012-1606. PMCID PMC4082824. https://resolver.caltech.edu/CaltechAUTHORS:20101214-101441084

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Abstract

Glypicans are conserved cell surface heparan sulfate proteoglycans expressed in a spatiotemporally regulated manner in many developing tissues including the nervous system. Here, we show that Glypican-1 (GPC1) is expressed by trigeminal placode cells as they ingress and contribute to trigeminal sensory neurons in the chick embryo. Either expression of full-length or truncated GPC1 in vivo causes defects in trigeminal gangliogenesis in a manner that requires heparan sulfate side chains. This leads to either abnormal placodal differentiation or organization, respectively, with near complete loss of the ophthalmic (OpV) trigeminal ganglion in the most severe cases after over-expression of full-length GPC1. Interestingly, modulating GPC1 alters levels of endogenous Wnt signaling activity in the forming trigeminal ganglion, as indicated by Wnt-reporter expression. Accordingly, GPC1 over-expression phenocopies Wnt inhibition in causing loss of OpV placodal neurons. Furthermore, increased Wnt activity rescues the effects of GPC1 over-expression. Taken together, these results suggest that appropriate levels of GPC1 are essential for proper regulation of canonical Wnt signaling during differentiation and organization of trigeminal placodal cells into ganglia.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2010.09.017 DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4082824/PubMed CentralArticle
ORCID:
AuthorORCID
Shiau, Celia E.0000-0002-9347-9158
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2010 Published by Elsevier Inc. Received 24 May 2010; revised 15 September 2010; accepted 21 September 2010. Available online 27 September 2010. We thank Andy Groves for the pTOP-nDSRed2 plasmid and members of M.B.-F. lab for discussions and technical advice, in particular Meyer Barembaum, Tatjana Sauka-Spengler, and Pablo Strobl. This work was supported by US National Institutes of Health (NIH) National Research Service Award 5T32 GM07616 to C. E. S. and N.H. and NIH grant DE16459 to M.B.-F.
Funders:
Funding AgencyGrant Number
NIH5T32 GM07616
NIHDE16459
Subject Keywords:Glypican; Trigeminal ganglion; Placode; Wnt
Issue or Number:1
PubMed Central ID:PMC4082824
Record Number:CaltechAUTHORS:20101214-101441084
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20101214-101441084
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21350
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Dec 2010 19:59
Last Modified:03 Oct 2019 02:23

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