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Comprehensive Analysis of CLE Polypeptide Signaling Gene Expression and Overexpression Activity in Arabidopsis

Jun, JiHyung and Fiume, Elisa and Roeder, Adrienne H. K. and Meng, Ling and Sharma, Vijay K. and Osmont, Karen S. and Baker, Catherine and Ha, Chan Man and Meyerowitz, Elliot M. and Feldman, Lewis J. and Fletcher, Jennifer C. (2010) Comprehensive Analysis of CLE Polypeptide Signaling Gene Expression and Overexpression Activity in Arabidopsis. Plant Physiology, 154 (4). pp. 1721-1736. ISSN 0032-0889. PMCID PMC2996011. doi:10.1104/pp.110.163683.

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Intercellular signaling is essential for the coordination of growth and development in higher plants. Although hundreds of putative receptors have been identified in Arabidopsis (Arabidopsis thaliana), only a few families of extracellular signaling molecules have been discovered, and their biological roles are largely unknown. To expand our insight into the developmental processes potentially regulated by ligand-mediated signal transduction pathways, we undertook a systematic expression analysis of the members of the Arabidopsis CLAVATA3/ESR-RELATED (CLE) small signaling polypeptide family. Using reporter constructs, we show that the CLE genes have distinct and specific patterns of promoter activity. We find that each Arabidopsis tissue expresses at least one CLE gene, indicating that CLE-mediated signaling pathways are likely to play roles in many biological processes during the plant life cycle. Some CLE genes that are closely related in sequence have dissimilar expression profiles, yet in many tissues multiple CLE genes have overlapping patterns of promoter-driven reporter activity. This observation, plus the general absence of detectable morphological phenotypes in cle null mutants, suggest that a high degree of functional redundancy exists among CLE gene family members. Our work establishes a community resource of CLE-related biological materials and provides a platform for understanding and ultimately manipulating many different plant signaling systems.

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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2010 American Society of Plant Biologists. Received July 30, 2010; accepted September 30, 2010; published September 30, 2010. Some figures in this article are displayed in color online but in black and white in the print edition. The online version of this article contains Web-only data. Open Access articles can be viewed online without a subscription. This work was supported by the National Science Foundation (Arabidopsis 2010 grant no. MCB 0313546). We thank Niki Kubat, Vicky Chen, and Liz Fong for assistance handling plants and generating constructs; the RIKEN, SAIL, GABI-KAT, and SALK collections for supplying indexed insertion mutant lines; and Sheila McCormick and Barbara Baker for helpful discussions.
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Issue or Number:4
PubMed Central ID:PMC2996011
Record Number:CaltechAUTHORS:20101220-135119606
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Official Citation:JiHyung Jun, Elisa Fiume, Adrienne H.K. Roeder, Ling Meng, Vijay K. Sharma, Karen S. Osmont, Catherine Baker, Chan Man Ha, Elliot M. Meyerowitz, Lewis J. Feldman, and Jennifer C. Fletcher. Comprehensive Analysis of CLE Polypeptide Signaling Gene Expression and Overexpression Activity in Arabidopsis. Plant Physiol. 154: 1721-1736; Plant Physiol. First published on December 1, 2010; 10.1104/pp.110.163683
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21447
Deposited By: Tony Diaz
Deposited On:05 Jan 2011 19:37
Last Modified:09 Nov 2021 15:57

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