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A wide field-of-view microscope based on holographic focus grid

Wu, Jigang and Cui, Xiquan and Zheng, Guoan and Lee, Lap Man and Yang, Changhuei (2010) A wide field-of-view microscope based on holographic focus grid. In: Three-dimensional and multidimensional microscopy : image acquisition and processing XVII. Proceedings of SPIE. No.7570. Society of Photo-optical Instrumentation Engineers , Bellingham, WA. ISBN 978-0-8194-7966-2.

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We have developed a novel microscope technique that can achieve wide field-of-view (FOV) imaging and yet possess resolution that is comparable to conventional microscope. The principle of wide FOV microscope system breaks the link between resolution and FOV magnitude of traditional microscopes. Furthermore, by eliminating bulky optical elements from its design and utilizing holographic optical elements, the wide FOV microscope system is more cost-effective. In our system, a hologram was made to focus incoming collimated beam into a focus grid. The sample is put in the focal plane and the transmissions of the focuses are detected by an imaging sensor. By scanning the incident angle of the incoming beam, the focus grid will scan across the sample and the time-varying transmission can be detected. We can then reconstruct the transmission image of the sample. The resolution of microscopic image is limited by the size of the focus formed by the hologram. The scanning area of each focus spot is determined by the separation of the focus spots and can be made small for fast imaging speed. We have fabricated a prototype system with a 2.4-mm FOV and 1-μm resolution. The prototype system was used to image onion skin cells for a demonstration. The preliminary experiments prove the feasibility of the wide FOV microscope technique, and the possibility of a wider FOV system with better resolution.

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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2010 SPIE.
Subject Keywords:Holographic optical element; Scanning microscope; Biomedical imaging
Series Name:Proceedings of SPIE
Issue or Number:7570
Record Number:CaltechAUTHORS:20110106-091729873
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Official Citation:Jigang Wu ; Xiquan Cui ; Guoan Zheng ; Lap Man Lee and Changhuei Yang "A wide field-of-view microscope based on holographic focus grid", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 757009 (February 24, 2010); doi:10.1117/12.842303;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21617
Deposited By: Tony Diaz
Deposited On:25 Jan 2011 18:05
Last Modified:09 Mar 2020 13:19

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