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In Vivo Assembly of an Archaeal Virus Studied with Whole-Cell Electron Cryotomography

Fu, Chi-yu and Wang, Kang and Gan, Lu and Lanman, Jason and Khayat, Reza and Young, Mark J. and Jensen, Grant J. and Doerschuk, Peter C. and Johnson, John E. (2010) In Vivo Assembly of an Archaeal Virus Studied with Whole-Cell Electron Cryotomography. Structure, 18 (12). pp. 1579-1586. ISSN 0969-2126. PMCID PMC3042139. http://resolver.caltech.edu/CaltechAUTHORS:20110113-115948690

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Abstract

We applied whole-cell electron cryotomography to the archaeon Sulfolobus infected by Sulfolobus turreted icosahedral virus (STIV), which belongs to the PRD1-Adeno lineage of dsDNA viruses. STIV infection induced the formation of pyramid-like protrusions with sharply defined facets on the cell surface. They had a thicker cross-section than the cytoplasmic membrane and did not contain an exterior surface protein layer (S-layer). Intrapyramidal bodies often occupied the volume of the pyramids. Mature virions, procapsids without genome cores, and partially assembled particles were identified, suggesting that the capsid and inner membrane coassemble in the cytoplasm to form a procapsid. A two-class reconstruction using a maximum likelihood algorithm demonstrated that no dramatic capsid transformation occurred upon DNA packaging. Virions tended to form tightly packed clusters or quasicrystalline arrays while procapsids mostly scattered outside or on the edges of the clusters. The study revealed vivid images of STIV assembly, maturation, and particle distribution in cell.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.str.2010.10.005DOIArticle
http://www.sciencedirect.com/science/article/B6VSR-51N1K32-7/2/15e9abe5829502ebe8687c9b351f7894PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042139/PubMed CentralArticle
ORCID:
AuthorORCID
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2010 Elsevier Ltd. Received: October 23, 2009; Revised: September 22, 2010; Accepted: October 1, 2010; Published: December 7, 2010. This work was supported by the National Institutes of Health (NIH) grant GM54076 to J.E.J., the NIH grant GM082545 to G.J.J. and the National Science Foundation (NSF) grants 0735297 and 0836656 to P.C.D., the NSF grant EF0802200 to M.J.Y., and gifts to Caltech from the Gordon and Betty Moore Foundation.
Funders:
Funding AgencyGrant Number
NIHGM54076
NIHGM082545
NSF0735297
NSF0836656
NSFEF0802200
Gordon and Betty Moore FoundationUNSPECIFIED
PubMed Central ID:PMC3042139
Record Number:CaltechAUTHORS:20110113-115948690
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110113-115948690
Official Citation:Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson, In Vivo Assembly of an Archaeal Virus Studied with Whole-Cell Electron Cryotomography, Structure, Volume 18, Issue 12, 8 December 2010, Pages 1579-1586, ISSN 0969-2126, DOI: 10.1016/j.str.2010.10.005. (http://www.sciencedirect.com/science/article/B6VSR-51N1K32-7/2/15e9abe5829502ebe8687c9b351f7894)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21750
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:14 Jan 2011 16:08
Last Modified:22 Oct 2015 21:10

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