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Iron-based ferritin nanocore as a contrast agent

Sana, Barindra and Johnson, Eric and Sheah, Kenneth and Poh, Chueh Loo and Lim, Sierin (2010) Iron-based ferritin nanocore as a contrast agent. Biointerphases, 5 (3). FA48 -FA52. ISSN 1559-4106.

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Self-assembling protein cages have been exploited as templates for nanoparticle synthesis. The ferritin molecule, a protein cage present in most living systems, stores excess soluble ferrous iron in the form of an insoluble ferric complex within its cavity. Magnetic nanocores formed by loading excess iron within an engineered ferritin from Archaeoglobus fulgidus (AfFtn-AA) were studied as a potential magnetic resonance (MR) imaging contrast agent. The self-assembly characteristics of the AfFtn-AA were investigated using dynamic light scattering technique and size exclusion chromatography. Homogeneous size distribution of the assembled nanoparticles was observed using transmission electron microscopy. The magnetic properties of iron-loaded AfFtn-AA were studied using vibrating sample magnetometry. Images obtained from a 3.0 T whole-body MRI scanner showed significant brightening of T1 images and signal loss of T2 images with increased concentrations of iron-loaded AfFtn-AA. The analysis of the MR image intensities showed extremely high R2 values (5300 mM^(−1) s^(−1)) for the iron-loaded AfFtn-AA confirming its potential as a T2 contrast agent.

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Additional Information:© 2010 American Vacuum Society. Received 17 May 2010; accepted 3 August 2010; published 14 December 2010. This paper is part of an In Focus section on Biointerphase Science in Singapore, sponsored by Brukner Optik Southeast Asia, IMRE, the Provost’s Office and School of Materials Science and Engineering of Nanyang Technological University, and Analytical Technologies Pte Ltd. The authors wish to thank Alasdair W. McDowall for assistance with electron microscopy, Dhandie Cervantes, and Renuka for assistance with the MRI. A.W.M. and the EM center are supported by funding awarded through the Beckman Institute Foundation. This work is funded by the Singapore Ministry of Education Academic Research Fund Tier I (Grant No. RG33/07).
Funding AgencyGrant Number
Beckman Institute FoundationUNSPECIFIED
Singapore Ministry of Education Academic Research FundRG33/07
Subject Keywords:biomedical materials, biomedical MRI, chromatography, light scattering, magnetic particles, magnetometers, nanobiotechnology, nanoparticles, proteins, self-assembly, transmission electron microscopy, Protein cage, Ferritin, Metallic core, MRI, Iron oxide
Issue or Number:3
Classification Code:PACS: 87.85.J-; 87.85.Rs; 87.61.-c; 75.50.Tt; 75.75.-c; 87.14.E-; 81.16.Dn
Record Number:CaltechAUTHORS:20110201-112655665
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Official Citation:Sana, B., E. Johnson, et al. (2010). "Iron-based ferritin nanocore as a contrast agent." Biointerphases 5(3): FA48-FA52.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21950
Deposited By: Tony Diaz
Deposited On:14 Feb 2011 17:51
Last Modified:03 Oct 2019 02:32

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