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B-Function Expression in the Flower Center Underlies the Homeotic Phenotype of Lacandonia schismatica (Triuridaceae)

Álvarez-Buylla, Elena R. and Ambrose, Barbara A. and Flores-Sandoval, Eduardo and Englund, Marie and Garay-Arroyo, Adriana and Garcıá-Ponce, Berenice and de la Torre-Bárcena, Eduardo and Espinosa-Matıás, Silvia and Martínez, Esteban and Piñeyro-Nelson, Alma and Engström, Peter and Meyerowitz, Elliot M. (2010) B-Function Expression in the Flower Center Underlies the Homeotic Phenotype of Lacandonia schismatica (Triuridaceae). Plant Cell, 22 (11). pp. 3543-3559. ISSN 1040-4651. PMCID PMC3015125. https://resolver.caltech.edu/CaltechAUTHORS:20110202-114241501

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Abstract

Spontaneous homeotic transformations have been described in natural populations of both plants and animals, but little is known about the molecular-genetic mechanisms underlying these processes in plants. In the ABC model of floral organ identity in Arabidopsis thaliana, the B- and C-functions are necessary for stamen morphogenesis, and C alone is required for carpel identity. We provide ABC model-based molecular-genetic evidence that explains the unique inside-out homeotic floral organ arrangement of the monocotyledonous mycoheterotroph species Lacandonia schismatica (Triuridaceae) from Mexico. Whereas a quarter million flowering plant species bear central carpels surrounded by stamens, L. schismatica stamens occur in the center of the flower and are surrounded by carpels. The simplest explanation for this is that the B-function is displaced toward the flower center. Our analyses of the spatio-temporal pattern of B- and C-function gene expression are consistent with this hypothesis. The hypothesis is further supported by conservation between the B-function genes of L. schismatica and Arabidopsis, as the former are able to rescue stamens in Arabidopsis transgenic complementation lines, and Ls-AP3 and Ls-PI are able to interact with each other and with the corresponding Arabidopsis B-function proteins in yeast. Thus, relatively simple molecular modifications may underlie important morphological shifts in natural populations of extant plant taxa.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.109.069153 DOIArticle
http://www.plantcell.org/cgi/content/abstract/22/11/3543PublisherArticle
http://dx.doi.org/10.1105/tpc.111.230160DOICorrection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3015125/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2010 American Society of Plant Biologists. Received June 16, 2009; revised October 5, 2010; accepted November 14, 2010; published November 30, 2010. Some figures in this article are displayed in color online but in black and white in the print edition. Online version contains Web-only data. Open Access articles can be viewed online without a subscription. This article is dedicated to the indigenous and peasant communities settled in the Mexican Lacandon rainforest, on whose well-being the conservation of this unique and diverse ecosystem depends. We hope this article calls attention to the importance of supporting these poor communities and the conservation of the Lacandon rainforest. The Frontera Corozal Cho'l community, as well as the Lacandon people, are acknowledged for authorizations and help during L. schismatica collection trips over the last 13 years. Special thanks to Ambrosio and his family and to Don Pascual for all their help and guidance while searching for L. schismatica populations. We thank John L. Bowman for discussions and help in the lab and field work and Stefan de Folter for advice and materials for the yeast assays. Rigoberto Vicencio Pérez-Ruiz helped with various laboratory tasks and Diana Romo with logistical support. Judith Márquez and Sonia Vázquez Santana were helpful during the early stages of this project. Financial support was from a Human Frontiers Science Program grant to E.M. and E.R.A.-B., from “Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica,” Universidad Nacional Autónoma de México (UNAM; IN230002, IN223607, IN229009-3, and IX207104 to E.R.A.-B., IN210408 to B.G.-P., and IN226510-3 to A.G.-A.), University of California-MEXUS ECO IE 271, Consejo Nacional de Ciencia y Tecnología (CONACYT) CO1.41848/A-1, 81542, and CO1.0435.B-1, and Redes temáticas de CONACYT de investigación en la red: Complejidad, Ciencia y Sociedad Grants to E.R.A.-B., 81433 to B.G.-P., and 90565 to A.G.-A. The CONACYT granted PhD scholarships to F.V.-S., A.P.-N., and E.F.-S. and an undergraduate scholarship to E.T.-B. A.P.-N.’s PhD research is done at the Posgrado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (Instituto de Ecología). B.A.A. was supported by National Science Foundation and UNAM fellowships.
Funders:
Funding AgencyGrant Number
Human Frontiers Science ProgramUNSPECIFIED
Universidad Nacional Autonoma de Mexico (UNAM)IN230002
Universidad Nacional Autonoma de Mexico (UNAM)IN223607
Universidad Nacional Autonoma de Mexico (UNAM)IN229009-3
Universidad Nacional Autonoma de Mexico (UNAM)IX207104
Universidad Nacional Autonoma de Mexico (UNAM)IN210408
Universidad Nacional Autonoma de Mexico (UNAM)IN226510-3
University of California-MEXUSECO IE 271
Consejo Nacional de Ciencia y Tecnologia (CONACYT)CO1.41848/A-1
Consejo Nacional de Ciencia y Tecnologia (CONACYT)81542
Consejo Nacional de Ciencia y Tecnologia (CONACYT)CO1.0435.B-1
Redes tematicas de CONACYT de investigacion en la red: Complejidad, Ciencia y Sociedad81433
Redes tematicas de CONACYT de investigacion en la red: Complejidad, Ciencia y Sociedad90565
NSFUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC3015125
Record Number:CaltechAUTHORS:20110202-114241501
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110202-114241501
Official Citation:Elena R. Álvarez-Buylla, Barbara A. Ambrose, Eduardo Flores-Sandoval, Marie Englund, Adriana Garay-Arroyo, Berenice García-Ponce, Eduardo de la Torre-Bárcena, Silvia Espinosa-Matías, Esteban Martínez, Alma Piñeyro-Nelson, Peter Engström, and Elliot M. Meyerowitz B-Function Expression in the Flower Center Underlies the Homeotic Phenotype of Lacandonia schismatica (Triuridaceae) PLANT CELL 2010 22: 3543-3559.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:21969
Collection:CaltechAUTHORS
Deposited By: Benjamin Perez
Deposited On:02 Feb 2011 23:39
Last Modified:03 Oct 2019 02:32

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