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Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis

Zilles, Julie L. and Croal, Laura R. and Downs, Diana M. (2000) Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis. Journal of Bacteriology, 182 (19). pp. 5606-5610. ISSN 0021-9193.

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Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.

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Additional Information:Copyright © 2000, American Society for Microbiology. Received 17 April 2000/Accepted 17 July 2000 We thank T. Begley for providing us with bacimethrin and for helpful discussion and S. Weber for technical assistance. Plasmid pVJS717 was obtained from V. Stewart. This work was supported by competitive grant MCB9723830 from the National Science Foundation and a Shaw Scientists Award from the Milwaukee Foundation. J.L.Z. was supported by a National Science Foundation Graduate Fellowship and a Wisconsin Alumni Research Foundation Annual Fellowship.
Issue or Number:19
Record Number:CaltechAUTHORS:ZILjbact00
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2199
Deposited By: Archive Administrator
Deposited On:14 Mar 2006
Last Modified:02 Oct 2019 22:51

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