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Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Estrozi, Leandro F. and Boehringer, Daniel and Shan, Shu-ou and Ban, Nenad and Schaffitzel, Christiane (2011) Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor. Nature Structural & Molecular Biology, 18 (1). pp. 88-90. ISSN 1545-9985. PMCID PMC3764645.

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We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

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Shan, Shu-ou0000-0002-6526-1733
Ban, Nenad0000-0002-9527-210X
Additional Information:© 2010 Nature Publishing Group, a division of Macmillan Publishers Limited. Received 25 April; accepted 8 October; published online 12 December 2010. We would like to thank G. Schoehn for collecting EM data, I. Berger for designing scSRP, C. Frick for technical assistance and X. Zhang for helpful suggestions. W. Wintermeyer (Max Planck Institute for Biophysical Chemistry) kindly provided pET24a-Ffh. J. Luirink (Vrije Universiteit Amsterdam) is acknowledged for providing pET9-FtsY. This work was supported by the Swiss National Science Foundation (SNSF) and the National Center of Excellence in Research Structural Biology program of the SNSF. The authors acknowledge support by the Electron Microscopy of ETH Zurich (EMEZ) and from the infrastructure of the Partnership for Structural Biology in Grenoble. Author Contributions: C.S. conceived the scSRP construct and performed sample preparations; S.-o.S. performed activity assays; D.B. did the electron microscopy; D.B., L.F.E. and C.S. performed the image analysis and model building; C.S., N.B., D.B. and S.-o.S. prepared the manuscript.
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)UNSPECIFIED
Electron Microscopy of ETH Zurich (EMEZ)UNSPECIFIED
Partnership for Structural Biology in GrenobleUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC3764645
Record Number:CaltechAUTHORS:20110214-091234951
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22157
Deposited By: Tony Diaz
Deposited On:10 Mar 2011 00:26
Last Modified:09 Mar 2020 13:18

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