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A 3-D microfluidic combinatorial cell array

Liu, Mike C. and Tai, Yu-Chong (2011) A 3-D microfluidic combinatorial cell array. Biomedical Microdevices, 13 (1). pp. 191-201. ISSN 1387-2176 .

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We present the development of a three-dimensional (3-D) combinatorial cell culture array device featured with integrated three-input, eight-output combinatorial mixer and cell culture chambers. The device is designed for cell-based screening of multiple compounds simultaneously on a microfluidic platform. The final assembled device is composed of a porous membrane integrated in between a Parylene 3-D microfluidic chip and a PDMS microfluidic chip. The membrane turned the cell culture chambers into two-level configuration to facilitate cell loading and to maintain cells in a diffusion dominated space during device operation. Experimentally, we first characterized the combined compound concentration profile at each chamber using a fluorescence method. We then successfully demonstrated the functionality of the quantitative cell-based assay by culturing B35 rat neuronal cells on this device and screening the ability of three compounds (1,5-dihydroxyisoquinoline, deferoxamine, and 3-aminobenzoic acid) to attenuate cell death caused by cytotoxic hydrogen peroxide. In another experiment, we assayed for the combinatorial effects of three chemotherapeutic compound exposures (vinorelbine, paclitaxel, and γ-linolenic acid) on human breast cancer cells, MDA-MB-231. The same technology will enable the construction of inexpensive lab-on-a-chip devices with high-input combinatorial mixer for performing high-throughput cell-based assay and highly parallel and combinatorial chemical or biochemical reactions.

Item Type:Article
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Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2010 Springer Science+Business Media, LLC. Published online: 10 November 2010. This work was supported by NSF Center for Embedded Network Sensing (CCR-0120778). The authors would like to thank Trevor Roper and members of the Caltech Micromachining Laboratory for fabrication assistance.
Funding AgencyGrant Number
Subject Keywords:3-D microfluidics Cell-based assay. Parylene Combinatorial mixer
Issue or Number:1
Record Number:CaltechAUTHORS:20110223-093810992
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Official Citation:Liu, M.C. & Tai, YC. Biomed Microdevices (2011) 13: 191. doi:10.1007/s10544-010-9484-4
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22446
Deposited By: Ruth Sustaita
Deposited On:23 Feb 2011 18:33
Last Modified:03 Oct 2019 02:37

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