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Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts

Lin, Ching-Shwun and Aebersold, Ruedi H. and Kent, Stephen B. H. and Varma, Madhu and Leavitt, John (1988) Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts. Molecular And Cellular Biology, 8 (11). pp. 4659-4668. ISSN 1098-5549. PMCID PMC365555. doi:10.1128/mcb.8.11.4659.

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The phosphoprotein plastin was originally identified as an abundant transformation-induced polypeptide of chemically transformed neoplastic human fibroblasts. This abundant protein is normally expressed only in leukocytes, suggesting that it may play a role in hemopoietic cell differentiation. Protein microsequencing of plastin purified from leukemic T lymphocytes by high-resolution two-dimensional gel electrophoresis produced eight internal oligopeptide sequences. An oligodeoxynucleotide probe corresponding to one of the oligopeptides was used to clone cDNAs from transformed human fibroblasts that encoded the seven other oligopeptides predicted for human plastin. Sequencing and characterization of two cloned cDNAs revealed the existence of two distinct, but closely related, isoforms of plastin--l-plastin, which is expressed in leukocytes and transformed fibroblasts, and t-plastin, which is expressed in normal cells of solid tissues and transformed fibroblasts. The leukocyte isoform l-plastin is expressed in a diverse variety of human tumor cell lines, suggesting that it may be involved in the neoplastic process of some solid human tumors.

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Additional Information:© 1988 by the American Society for Microbiology. Received 14 June 1988/Accepted 5 August 1988 Most of the work performed by C.-S.L., M.V., and J.L. was carried out at the Linus Pauling Institute, Palo Alto, Calif. We thank Sandra Schwoebel (Linus Pauling Institute) for help in the preparation of this manuscript and Wade Hines (California Institute of Technology) for expert technical assistance. This work was supported by Public Health Service grant CA-34763 (to J.L.) from the National Institutes of Health and by grants from the Biological Instrumentation Division of the National Science Foundation and the Monsanto Corporation. R.H.A. was the recipient of a fellowship from the Swiss National Science Foundation.
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Monsanto CorporationUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC365555
Record Number:CaltechAUTHORS:LINmcb88
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ID Code:2251
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Deposited On:19 Mar 2006
Last Modified:08 Nov 2021 19:46

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