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Correlated Light and Electron Cryo-Microscopy

Briegel, Ariane and Chen, Songye and Koster, Abraham J. and Plitzko, Jürgen M. and Schwartz, Cindi L. and Jensen, Grant J. (2010) Correlated Light and Electron Cryo-Microscopy. In: Cryo-EM, Part A: Sample Preparation and Data Collection. Methods in Enzymology. No.481. Academic Press , Amsterdam, pp. 317-341. ISBN 9780123749062.

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Light and electron cryo-microscopy have each proven to be powerful tools to study biological structures in a near-native state. Light microscopy provides important localization information, while electron microscopy provides the resolution necessary to resolve fine structural details. Imaging the same sample by both light and electron cryo-microscopy is a powerful new approach that combines the strengths of both techniques to provide novel insights into cellular ultrastructure. In this chapter, the methods and instrumentation currently used to correlate light and electron cryo-microscopy are described in detail.

Item Type:Book Section
Related URLs:
URLURL TypeDescription DOIArticle
Briegel, Ariane0000-0003-3733-3725
Chen, Songye0000-0001-5407-5049
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2010 Elsevier Inc. Available online 29 September 2010. We thank Dr. Alasdair McDowall for help with correlated FLM/ECM at Caltech. Work at Caltech was supported by the HHMI, NIH grants R01 AI067548 and P01 GM066521 to GJJ, and gifts from the Gordon and Betty Moore Foundation. Work at Boulder was supported by NIH Research Resource Grant #RR00592 to A. Hoenger. Work at the Max-Planck-Institute of Biochemistry in Martinsried was supported by the European Commission’s 7th Framework Programme (grant agreement HEALTH-F4-2008-201648/ PROSPECTS).
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHR01 AI067548
NIHP01 GM066521
Gordon and Betty Moore FoundationUNSPECIFIED
European CommissionHEALTH-F4-2008-201648/PROSPECTS
Series Name:Methods in Enzymology
Issue or Number:481
Record Number:CaltechAUTHORS:20110228-111002641
Persistent URL:
Official Citation:Ariane Briegel, Songye Chen, Abraham J. Koster, Jurgen M. Plitzko, Cindi L. Schwartz, Grant J. Jensen, Correlated Light and Electron Cryo-Microscopy, In: Grant J. Jensen, Editor(s), Methods in Enzymology, Academic Press, 2010, Volume 481, Cryo-EM Part A Sample Preparation and Data Collection, Pages 317-341, ISSN 0076-6879, ISBN 9780123749062, DOI: 10.1016/S0076-6879(10)81013-4. (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22547
Deposited By: Tony Diaz
Deposited On:03 Mar 2011 19:33
Last Modified:04 Oct 2019 23:01

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