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Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle

Maltezos, George and Johnston, Matthew and Taganov, Konstantin and Srichantaratsamee, Chutatip and Gorman, John and Baltimore, David and Chantratita, Wasun and Scherer, Axel (2010) Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle. Applied Physics Letters, 97 (26). Art. No. 264101. ISSN 0003-6951. https://resolver.caltech.edu/CaltechAUTHORS:20110228-114858253

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Abstract

Thermal ramp rate is a major limiting factor in using real-time polymerase chain reaction (PCR) for routine diagnostics. We explored the limits of speed by using liquid for thermal exchange rather than metal as in traditional devices, and by testing different polymerases. In a clinical setting, our system equaled or surpassed state-of-the-art devices for accuracy in amplifying DNA/RNA of avian influenza, cytomegalovirus, and human immunodeficiency virus. Using Thermococcus kodakaraensis polymerase and optimizing both electrical and chemical systems, we obtained an accurate, 35 cycle amplification of an 85-base pair fragment of E. coli O157:H7 Shiga toxin gene in as little as 94.1 s, a significant improvement over a typical 1 h PCR amplification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1063/1.3530452DOIUNSPECIFIED
http://apl.aip.org/resource/1/applab/v97/i26/p264101_s1PublisherUNSPECIFIED
http://link.aip.org/link/doi/10.1063/1.3530452 PublisherUNSPECIFIED
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 2010 American Institute of Physics. Received 20 September 2010; accepted 5 November 2010; published online 28 December 2010. The authors gratefully acknowledge financial support from the Boeing Corporation under the SRDMA program and from the NIH under Grant No. R01 HG002644.
Funders:
Funding AgencyGrant Number
Boeing Corporation UNSPECIFIED
NIHR01 HG002644
Subject Keywords:biochemistry, biosensors, DNA, enzymes, heat transfer, lab-on-a-chip, microorganisms, patient diagnosis
Issue or Number:26
Classification Code:PACS: 87.85.fk; 87.15.R; 87.14.ej; 87.14.gk
Record Number:CaltechAUTHORS:20110228-114858253
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110228-114858253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22555
Collection:CaltechAUTHORS
Deposited By: Benjamin Perez
Deposited On:28 Feb 2011 22:20
Last Modified:03 Oct 2019 02:38

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